A unique combination of Alchornea cordifolia and Pterocarpus santalinoides in the management of multi-drug resistant diarrhoegenic bacterial infection.

Asian Journal of Biomedical and Pharmaceutical Sciences Pub Date : 2020-01-01 DOI:10.35841/2249-622X.69.5059

M. Obaji, I. Enweani, A. Oli

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引用次数: 1

Abstract

Background: Traditionally, diarrhoea in our locality has often been managed using herbs. Multi-drugresistant organisms refer tomicrobes that areresistant to the effects of antimicrobial drugs to which they were previously susceptible at therapeutic range. Objectives: This study investigates the combined effects of Alchornea cordifolia and Pterocarpus santalinoides leaves extracts against selected multi-drug resistant diarrhoegenic bacteria. Methods: Fresh plant seeds were collected from the plants, shade-dried, pulverized and extracted with methanol in a Soxhlet apparatus. Antibacterial activities of these plants were investigated using agarwell diffusion assay. The MIC and FIC of the extracts against test isolates were determined using agar well diffusion method and checkerboard assay respectively. The bactericidal effects of the extracts against the isolates were evaluated using Time-Kill Assay. Results: MIC values of methanolic extracts against drug resistant strains of Salmonella typhi , Shigella dysenteriae , Escherichia coli and Staphylococcus aureusranges from 6.25 µg/mL to 12.5 µg/mL. The combination of Alchornea cordifolia and Pterocarpus santalinoides extracts showed synergy at 8:2 and 4:6 combinations against S. aureus, Salmonella typhi and E. coli; at 7:3 combinations against E. coli ; at 6:4 and 3:7 combinations against S. aureus, Salmonella typhi , S. dysenteriae ; at 5:5 combinations against all the test isolates; at 2:8 combinations against S. aureus , S. dysenteriae and E. coli but additive against Salmonella organisms; at 1:9 combinations against S. aureus and Salmonella organism. Conclusion: The methanol extracts of the plants at combinations 5:5 is synergistic against all the tested multi-drug resistant diarrhoegenic bacteria. Combination of Salmonellaand A. cordifolia showed potentials of synergism against the test isolates. The detection of synergy suggests presence of antibiotic resistance modifying compounds in those extracts.

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在多重耐药腹泻性细菌感染的管理中，堇青花和山蕨的独特组合。

背景:传统上，我们当地的腹泻通常使用草药治疗。多重耐药生物是指对抗菌药物产生耐药性的微生物，而这些微生物以前在治疗范围内是敏感的。目的:研究菖蒲叶提取物联合抗多种耐药腹泻菌的作用。方法:采集新鲜植物种子，在索氏装置中遮荫干燥、粉碎、甲醇提取。用琼脂扩散法研究了这些植物的抑菌活性。分别用琼脂孔扩散法和棋盘法测定提取物对分离株的MIC和FIC。采用时间杀伤法评价提取物对分离菌株的杀菌效果。结果:甲醇提取物对伤寒沙门氏菌、痢疾志贺氏菌、大肠杆菌和金黄色葡萄球菌耐药菌株的MIC值在6.25µg/mL ~ 12.5µg/mL之间。菖蒲提取物与山菖蒲提取物联合对金黄色葡萄球菌、伤寒沙门氏菌和大肠杆菌的协同作用分别为8:2和4:6;对大肠杆菌的联合作用为7:3;金黄色葡萄球菌、伤寒沙门氏菌、痢疾沙门氏菌分别为6:4和3:7;针对所有测试分离株的组合比例为5:5;抗金黄色葡萄球菌、痢疾葡萄球菌和大肠杆菌的组合比例为2:8，但抗沙门氏菌的添加剂比例为2:8;金黄色葡萄球菌和沙门氏菌的比例为1:9。结论:组合5:5的甲醇提取物对所有耐多药腹泻菌均有协同作用。沙门氏菌与cordifolia联合对试验分离株具有协同作用。协同作用的检测表明这些提取物中存在抗生素抗性修饰化合物。

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Asian Journal of Biomedical and Pharmaceutical Sciences

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