Development and Validation of Competitive ELISA for Detection of H5 Hemagglutinin Antibodies

IF 3.9 3区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

World's Poultry Science Journal Pub Date : 2023-07-07 DOI:10.3390/poultry2030026

Orie Hochman, Wanhong Xu, Ming Yang, Chengbo Yang, A. Ambagala, A. Rogiewicz, Joseph J. Wang, Y. Berhane

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引用次数: 0

Abstract

Influenza A viruses (IAVs) belonging to the goose/Guangdong (Gs/GD)-lineage H5Nx remain a major concern for the global poultry industry, wildlife, and humans. The hemagglutinin (HA) protein is the dominant antigenic epitope carrier within IAV, which in turn triggers substantial immunogenic responses in the infected host. The current study describes the development and validation of a highly sensitive competitive H5 ELISA (cELISA) based on a novel monoclonal antibody developed in mice immunized with inactivated virus H5N1 (A/Turkey/ON/6213/1966). The cELISA is capable of detecting the H5 antibody response to a wider range of H5-subtype viruses belonging to both North American and Eurasia lineages, including the Gs/GD H5Nx from clade 2.3.4.4b that is currently causing the highly pathogenic avian influenza outbreaks in Eurasia, Africa, and Latin and North America. The developed H5 cELISA provides a specific, sensitive, and species-independent serological assay for the rapid detection of H5 antibodies. The assay is more robust and more sensitive than the hemagglutination inhibition assay, which is the “Gold standard”. The assay can be used in serological diagnosis, serosurveillance, and vaccine monitoring of serum samples collected from different species of animals.

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H5血凝素抗体竞争性ELISA检测方法的建立与验证

属于鹅/广东(Gs/GD)系H5Nx的甲型流感病毒(iav)仍然是全球家禽业、野生动物和人类关注的主要问题。血凝素(HA)蛋白是IAV中主要的抗原表位载体，进而在感染宿主中引发大量的免疫原性反应。目前的研究描述了一种高度敏感的竞争性H5 ELISA (cELISA)的开发和验证，该ELISA基于一种新型单克隆抗体，该抗体是在用灭活病毒H5N1 (a /Turkey/ on /6213/1966)免疫的小鼠中开发的。cELISA能够检测对属于北美和欧亚谱系的更广泛的H5亚型病毒的H5抗体反应，包括来自进化枝2.3.4.4b的Gs/GD H5Nx，该病毒目前正在欧亚大陆、非洲、拉丁美洲和北美引起高致病性禽流感爆发。开发的H5 cELISA为快速检测H5抗体提供了特异性、敏感性和物种无关的血清学分析。该试验比血凝抑制试验更可靠，更敏感，是“金标准”。该试验可用于从不同种类动物采集的血清样本的血清学诊断、血清监测和疫苗监测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World's Poultry Science Journal 农林科学-奶制品与动物科学

CiteScore

6.10

自引率

7.40%

发文量

审稿时长

12-24 weeks

期刊介绍： World''s Poultry Science Journal is the official publication of the World’s Poultry Science Association. The journal provides authoritative reviews in poultry science and an international forum for the exchange and dissemination of information including research, education and industry organisation. Each issue includes poultry industry-related news, regional reports on global developments in poultry, reports from specialist scientific working groups, book reviews, association news and a calendar of forthcoming events. Coverage includes breeding, nutrition, welfare, husbandry, production systems, processing, product development, physiology, egg and meat quality, industry structure, economics and education. The journal is of interest to academics, researchers, students, extension workers and commercial poultry producers.