Methicillin Resistant Staphylococcus aureus in Wound Swabs of Patients Attending a Public Hospital in Warri Delta State, Nigeria

O. Agbagwa, O. Edje
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Abstract

Aim: This study was carried out to isolate, identify and determining the prevalence of Methicillin Resistant Staphylococcus aureus from wound swabs of patients attending a public hospital in Warri, Delta state. aspect the while the second phase carried Methodology: A total of one hundred (100) wound swab samples were collected from patients in four (4) wards in a public hospital in Warri Delta state Nigeria. Samples were collected from male medical ward (MMW), male surgical ward (MSW), female medical ward (FMW) and female surgical ward (FSW). Sixty-eight (68) samples were from males and thirty-two (32) from females. The samples were evaluated using Mannitol salt agar and Oxoid BrillianceTM MRSA agar. The isolates were identified using morphology, colour indication, Gram reaction and biochemical tests. Antibiotic sensitivity was carried out using Kirby Bauer disc diffusion method. Results: The study showed that 52 samples had significant growth of Staphylococcus aureus while 48 had no significant growth of the same bacteria. Nineteen (19) out of the fifty-two were confirmed to be MRSA. Erythromycin was found to be the most sensitive drug after the antibiotics sensitivity test while cloxacillin and cefuroxime showed the highest resistance using Kirby Bauer' disc diffusion technique. This study showed that the prevalence of MRSA in the public hospital studied is 19%.
尼日利亚瓦里三角洲州一家公立医院住院患者伤口拭子中的耐甲氧西林金黄色葡萄球菌
目的:本研究旨在分离、鉴定和确定在三角洲州瓦里一家公立医院就诊的患者伤口拭子中耐甲氧西林金黄色葡萄球菌的流行情况。方法:从尼日利亚瓦里三角洲州一家公立医院的四(4)个病房的患者中收集了总共一百(100)份伤口拭子样本。样本采集于男内科病房(MMW)、男外科病房(MSW)、女内科病房(FMW)和女外科病房(FSW)。男性68份,女性32份。使用甘露醇盐琼脂和Oxoid BrillianceTM MRSA琼脂对样品进行评估。采用形态、显色、革兰氏反应和生化试验对分离菌株进行鉴定。采用Kirby Bauer圆盘扩散法进行抗生素敏感性检测。结果:研究表明,52份样品中金黄色葡萄球菌明显生长,48份样品中金黄色葡萄球菌无明显生长。52人中有19人被确诊为MRSA。采用Kirby Bauer盘片扩散法进行抗生素敏感性试验,发现红霉素是最敏感的药物,而氯西林和头孢呋辛的耐药性最高。本研究显示,MRSA在公立医院的患病率为19%。
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