Bacillus mojavensis: biofilm formation and biochemical investigation of its bioactive metabolites

Abstract

Bacillus mojavensis is an endophytic bacterium which has been reported to have fungicidal effect against some phytopathogens. Bioactive secondary metabolites produced by B. mojavensis could have promising applications in agricultural, food industry and clinical fields. The current research has been conducted to: i) evaluate the antagonistic effect of B. mojavensis isolate against some phytopathogens; ii) characterize chemically the principal bioactive substances produced by the studied isolate of B. mojavensis using Gas Chromatography-Mass Spectroscopy (GC-MS); iii) evaluate its ability to produce a biofilm using ELISA technique. Results showed that the studied isolate has an antagonistic activity against the majority of tested microorganisms. Results showed also that the studied isolated produced a biofilm in Supplemented Luria-Bertani Casamino acid (SLB) and Minimal Mineral (MM) medium. The substantial attached growth in SLB was significantly higher than MM media. GC-MS analysis revealed the presence of 9 compounds accounting 87.8% of the total extract, where oxygenated monoterpenes are the main constituents.