Screening and Identification of Actinomycetes from animals’ dung for antimicrobial activity

Abstract

The present study was searching for Actinomycetes isolates with antimicrobial activity from animals’ dung. Twenty-two Actinomycetes isolates were purified and nominated consistent with their culture features on starch nitrate agar medium. These purified isolates were surveyed for antimicrobial activity against different bacterial and fungal strains. Among all tested actinomycetes isolates, the isolate A12 had the highest antimicrobial activity. The A12 isolate's morphological, physiological, and biochemical data all pointed to it being a member of the Streptomyces genus. The 16S rRNA gene sequence and phylogenetic connection of strain A12 revealed that it belongs to the Streptomyces hypolithicus HSM#10. The optimum conditions of growth and antimicrobial activity were submerged cultivation, temperature 30 ᵒ C, pH 7 for 7 days. Starch and potassium nitrate as best carbon and nitrogen source respectively and1% (w/v) NaCl. Ethyl acetate was used to extract antimicrobial metabolites from the isolate A12, which were then examined using thin layer chromatography (TLC)