An electrochemical DNA-Prussian blue-carbon paste biosensor for the detection of ascorbic acid in pharmaceuticals

2014 International Conference on Control, Instrumentation, Communication and Computational Technologies (ICCICCT) Pub Date : 2014-07-10 DOI:10.1109/ICCICCT.2014.6992929

R. Rimal Isaac, U. Manjusree Nair, P. Praseetha

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引用次数: 1

Abstract

A biosensor for the detection of ascorbic acid is developed by preparing a carbon paste electrode (CPE) electrochemically deposited with Prussian blue (PB) and DNA adsorbed on its surface. Cyclic voltammetry (CV) was used to study the electrochemical properties of the biosensor. The successful electro-deposition of PB was confirmed using Raman Spectroscopy. From the results it was concluded that PB acts as a redox mediator. The sensing of ascorbic acid was based on the DNA damage induced by hydroxyl radical generated by Fenton reaction; ascorbic acid effectively scavenges the hydroxyl radical. The amount of DNA damage is proportional to the amount of ascorbic acid concentration which is analyzed by Cyclic Voltammetry. The results show that the biosensor can be used to measure ascorbic acid in pharmaceutical tablets. The detection range of the biosensor is 0.2 mg/L to 2.2 mg/L. The effect of interference by some common compounds used in pharmaceutical formulations and the stability of the biosensor were studied. The results indicate the biosensor has high sensitivity and selectivity in the detection of ascorbic acid in tablets.

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用于药物中抗坏血酸检测的电化学dna -普鲁士蓝碳糊生物传感器

制备了一种用于抗坏血酸检测的碳糊电极(CPE)，并在CPE表面吸附了DNA和普鲁士蓝。利用循环伏安法(CV)研究了该生物传感器的电化学性能。利用拉曼光谱证实了PB电沉积的成功。结果表明，PB是一种氧化还原介质。抗坏血酸的检测是基于Fenton反应产生的羟基自由基对DNA的损伤;抗坏血酸能有效清除羟基自由基。DNA损伤量与抗坏血酸浓度成正比，用循环伏安法分析。结果表明，该传感器可用于药物片剂中抗坏血酸的测定。该生物传感器检测范围为0.2 mg/L ~ 2.2 mg/L。研究了药物制剂中常用化合物的干扰作用和生物传感器的稳定性。结果表明，该传感器对片剂中抗坏血酸的检测具有较高的灵敏度和选择性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2014 International Conference on Control, Instrumentation, Communication and Computational Technologies (ICCICCT)

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