Some Barcoding DNA Sequence Analysis of Sphagnum nepalense H. Suzuki, a Bryophyte Species Endemic to East Nepal

Journal of Plant Resources and Environment Pub Date : 2022-12-31 DOI:10.3126/bdpr.v20i01.56577

Madhu Shudan Thapa Magar, Seerjana Maharjan, Januka Pathak, D. Kandel, Ganga Rijal

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Abstract

Sphagnum nepalense is a bryophyte endemic to Nepal. The objective of the present study is to analyze DNA barcoding markers useful for delineating the Sphagnum species. Here, a specimen of Sphagnum nepalense collected from the bank of Maipokhari lake, Ilam (2107 m asl) was used. Three chloroplast loci from the sample viz. rbcL, psbA-trnH and trnF-trnL, the latter two being intergenic spacers, were amplified and sequenced. Four accessions of plastome sequences of S. junghuhnianum, S. multifibrosum, S. palustre and S. subsecundum were retrieved from the National Center for Biotechnology Information (NCBI). Evolutionary analysis was performed following the Maximum Likelihood approach using MEGA X. The result showed that the evolutionary tree generated with single locus trnF-trnL and combined sequences of trnF-trnL and psbA-trnH was better compared to that generated with the sequence of other single locus and even the combined sequence of rbcL, psbA-trnH and trnF-trnL. The sequence data generated in this study for Sphagnum nepalense are novel to the scientific community.

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尼泊尔东部特有苔藓植物尼泊尔Sphagnum H. Suzuki的条形码DNA序列分析

尼泊尔Sphagnum nepalense是尼泊尔特有的苔藓植物。本研究的目的是分析DNA条形码标记对泥鳅种类的描述有用。本研究使用的是一种从伊拉姆Maipokhari湖岸(海拔2107米)采集的尼泊尔泥鳅标本。对rbcL、psbA-trnH和trnF-trnL三个叶绿体位点进行扩增和测序，后两个位点为基因间间隔物。从美国国家生物技术信息中心(NCBI)检索了junghuhnium、S. multifibrosum、S. palustre和S. subsecundum的4个质体组序列。利用MEGA x软件采用极大似然方法进行进化分析，结果表明，单位点trnF-trnL和trnF-trnL与psbA-trnH组合序列生成的进化树比其他单位点乃至rbcL、psbA-trnH和trnF-trnL组合序列生成的进化树更好。本研究获得的尼泊尔泥鳅序列数据对科学界来说是新颖的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Plant Resources and Environment Agricultural and Biological Sciences-Plant Science

CiteScore

0.60

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0.00%

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