Antimicrobial activity of Antibiotics and Antiseptics (Dettol and Betadine) against Clinical Isolates of Pseudomonas aeruginosa

Abstract

Hundred samples viz. urine, blood, wound, pus and sputum collected from different patients were found to harbour Pseudomonas aeruginosa (27%) with a maximum isolation from wound samples (33.33%) and minimum from blood samples (11.11%). The degree of resistance of Pseudomonas aeruginosa isolates to different antibiotics like Ceftazidime (30μg), Amikacin (30μg), Imipenem (10μg), Ciprofloxacin (30μg), Tetracycline (30μg), Gentamicin (10μg), Norfloxacin (10μg), Penicillin (30μg), Chloramphenicol (30μg), and Ofloxacin (5μg) varied from 56% to 100%. Antiseptics i.e. Betadine and Dettol were found to be more effective against the MDR strain of Pseudomonas aeruginosa at the dilutions of 10 -1 and 10 -2 . Duration of the disease and hospitalization duration, evaluated as risk factors for Pseudomonas aeruginosa colonization were found to be statistically significant while age and gender were found to be statistically nonsignificant. The incidence of multidrug resistance of Pseudomonas aeruginosa is increasing fast due to the frequent use of antibiotics and antiseptics, which are used extensively in hospitals and healthcare centers, therefore it is a need to develop alternative antimicrobial agents for the treatment of infectious diseases. Key-wordsPseudomonas aeruginosa, Betadine and Dettol, Antibiotic, Antiseptic INTRODUCTION Pseudomonas aeruginosa is one of the leading causes of nosocomial infections, reported worldwide. The gram negative, rod shaped bacterium (0.5-0.8 m and 1.5-3.0 m in size) is ubiquitous with normal nutritional requirement and has emerged as the epitome of opportunistic pathogen of humans. There is hardly any tissue that it cannot infect, if the tissue defenses are compromised in some manner [1] . P. aeruginosa is commonly encountered in health-associated infections. Multiple surveillance programs have reported the organism as one of the leading causes of nosocomial infection. [2-4] According to Center for Disease Control (CDC), incidence of P. aeruginosa infections in U.S. hospitals averages about 0.4% (4 per 1000 discharges) and the bacterium is the fourth most commonly isolated nosocomial pathogen accounting for 10.1% of all hospital acquired infections [1] . Access this article online Quick Response Code Website: