The role of endothelin-1 in strain-related susceptibility to develop hypoxic pulmonary hypertension in rats

Respiration physiology Pub Date : 2001-11-01 DOI:10.1016/S0034-5687(01)00255-9

M.R. Karamsetty , L. Pietras , J.R. Klinger , J.J. Lanzillo , J.C. Leiter , L.-C. Ou , N.S. Hill

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引用次数: 5

Abstract

The Hilltop (H) strain compared to the Madison (M) strain of Sprague–Dawley rats develops severe pulmonary hypertension in response to chronic hypoxia. We tested the hypothesis that endothelin-1 (ET-1) contributes to these strain-related differences. Plasma ET-1 content was not modified by chronic hypoxia in either strain. The lung ET-1 peptide and preproET-1 mRNA content were significantly increased to the same magnitude in both strains at 2 and 3 weeks of hypoxia. The ET_A receptor mRNA increased more at 3 weeks of hypoxia in the lungs of H rats than in M rats, but not at other time points. The ET_B receptor mRNA was not modified by hypoxia in either strain. After 3 days of normoxic recovery following 2 weeks of hypoxia, ET-1 protein and mRNA levels decreased to baseline levels in both rat strains. We conclude that ET-1 does not contribute to the development of cardiopulmonary differences between the H and M strains in response to hypoxia.

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内皮素-1在大鼠缺氧性肺动脉高压易感性中的作用

Hilltop (H)毒株与Madison (M)毒株相比，Sprague-Dawley大鼠对慢性缺氧的反应出现严重的肺动脉高压。我们检验了内皮素-1 (ET-1)导致这些菌株相关差异的假设。血浆ET-1含量均未因慢性缺氧而改变。缺氧2周和3周时，两组小鼠肺ET-1肽和preproET-1 mRNA含量均显著升高至相同水平。缺氧3周时，H大鼠肺内ETA受体mRNA表达明显高于M大鼠，其他时间点无明显差异。两种菌株的ETB受体mRNA均未被缺氧修饰。缺氧2周后正常恢复3天后，两种大鼠的ET-1蛋白和mRNA水平均降至基线水平。我们得出结论，ET-1不促进H和M菌株对缺氧反应的心肺差异的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Respiration physiology

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