The Complete Genome Sequence of Transmissible Gastroenteritis Virus Strain SC-Y

Abstract

Porcine transmissble gastroenteritis (TGE) is a highly contagious enteric disease of swine caused by transmissible gastroenteritis virus (TGEV). The disease is especislly severe in piglets under two weeks of age, characterized by vomiting, diarrhea and nearly 100% mortality. In this study, the complete genome sequence is available from SC-Y strain isolated in Sichuan province. Sixteen overlapping fragments were amplified by RT-PCR, the amplified fragments were further cloned and sequenced. The size of complete genome is 28,590 nucleotides in length, including the poly (A) tail. NCBI accession number DQ44374. Phylogenetic tree analysis showed the SC-Y was a close evoluntionary relationship to the American Purdue cluster. There were three nucleotides ((ATC) deletions in ORF3a as markers of the Purdue strains. The research make us better understand the molecular structure and evolution of the TGEV strains prevalent in China.