Antifungal and antiparasitic activity of Wunderlichia azulensis (Asteraceae) roots

F. G. Trindade, G. Taveira, L. D. de Carvalho, V. F. Moreira, R. B. B. Feitoza, V. Gomes, I. Vieira, R. Braz-Filho, E. D. de Melo, M. da Cunha
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Abstract

Abstract The aim of this work was to evaluate the antifungal and antiparasitic activity of roots of Wunderlichia azulensis (Asteraceae). A CH2Cl2 partition of W. azulensis was tested against Candida tropicalis, C. buinensis and Trypanosoma cruzi parasites. A chemical study of the CH2Cl2 partition resulted in the isolation and identification of dehydrobrachylaenolide (1), lasiodiplodin (2), a taraxerane-type triterpene (3) and a mixture of sitosterol and stigmasterol (4–5). Of the 13 fractions tested, fractions 5 and 7 were the most bioactive ones. No live epimastigotes were observed even after the shortest time of exposure to fraction 7 (25 μg.mL−1/24 h). Fractions 5 and 7 inhibited yeast growth in proportions between 63.9–80.7% at 50 μg.mL−1, and 70.3–96.7% at 100 μg.mL−1. Fractions 5 and 7 (100 μg.mL−1) induced loss of viability of C. buinensis by 97.61 and 92.63%, respectively, whereas C. tropicalis was less susceptible. The SYTOX Green marker used on Candida yeasts suggested membrane permeabilization and consequently plasma membrane damage. We concluded that W. azulensis offers new sources of biologically-active components. However, complementary studies on other variables, such as synergy, toxicology, and mechanism of action, must be carried out.
紫纹立菌根的抗真菌和抗寄生虫活性
摘要:本文研究了菊科植物蓝纹立菌(Wunderlichia azulensis)根的抗真菌和抗寄生虫活性。用CH2Cl2分离体对热带假丝酵母、商业假丝酵母和克氏锥虫进行了抑菌试验。通过对CH2Cl2分割的化学研究,分离鉴定出脱氢brachylaenolide(1)、lasiodiplodin(2)、taraxerane型三萜(3)和谷甾醇和豆甾醇的混合物(4-5)。在13个组分中,组分5和组分7的生物活性最高。即使暴露时间最短(25 μg.mL−1/24 h),也未观察到活的附毛菌。馏分5和馏分7在50 μg时抑制酵母生长的比例在63.9-80.7%之间。100 μg.mL−1时,为70.3 ~ 96.7%。组分5和组分7 (100 μg.mL−1)对布氏棉铃虫活力损失的影响分别为97.61%和92.63%,而对热带棉铃虫的影响较小。用SYTOX绿色标记对念珠菌进行了膜渗透,从而导致了质膜损伤。由此得出结论,青霉提供了新的生物活性成分来源。然而,对其他变量的补充研究,如协同作用、毒理学和作用机制,必须进行。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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