Effect of frozen storage on microbial load of hybrid heteroclarias, Clarias gariepinus and Oreochromis niloticus

A. Ayeloja, W. Jimoh, M. O. Shittu, B. O. Batatunde
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Abstract

Effect of frozen storage on microbial load of hybrid Heteroclarias, Clarias gariepinus and Oreochromis niloticus was studied. Fifty samples, each of Heteroclarias, C. gariepinus and O. niloticus with an average weight of 210 + 15g were collected at a commercial fish farm in the study area after which they were processed and frozen at -18°C and microbial analyses were done at 0, 14, 28, 42 and 56 days after frozen storage. Data obtained were logarithmically transformed (log cfu/g) and then subjected to statistical analysis using SPSS 16.0 version. No significant (p>0.05) difference was found for total viable count (TVC), total fungal count (TFC), total coliform count (TCC) and Klebsiella spp. count of the fish species studied during the frozen period. The potential of freezing as a good fish preservation method was established as it inhibited microbial activities thereby elongating fish shelf life. It was concluded that Clarias gariepinus and Oreochromis niloticus fish species can be kept safe up to 56 days by freezing as the microbial loads did not change significantly during the period. Uncontrolled discharge of effluents into the surrounding water bodies should also be checked to avoid contamination prior to fish harvest.
冷冻贮藏对杂交种克拉尾鱼、加里宾克拉尾鱼和尼罗鱼微生物负荷的影响
研究了冷冻贮藏对杂交异Clarias、Clarias gariepinus和Oreochromis niloticus微生物负荷的影响。在研究区某商业养鱼场采集了平均体重210 + 15g的异克拉尾鱼、加里平尾鱼和尼罗尾鱼各50只,处理后在-18°C冷冻,并在冷冻后0、14、28、42和56 d进行微生物分析。得到的数据进行对数变换(log cfu/g),然后使用SPSS 16.0版本进行统计分析。冷冻期鱼类总活菌数(TVC)、总真菌数(TFC)、总大肠菌群数(TCC)和克雷伯氏菌数均无显著差异(p>0.05)。冷冻作为一种良好的鱼类保存方法的潜力被确立,因为它可以抑制微生物活动,从而延长鱼类的保质期。综上所述,在此期间,由于微生物负荷变化不明显,因此,加里雅那克拉丽鱼和尼罗褐口鱼的冷冻保存时间可达56 d。在捕鱼前,亦应检查无节制地向周围水体排放污水,以避免污染。
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