Erythrocyte interaction with titanium nanostructured surfaces.

IF 2.4
In vitro models Pub Date : 2022-08-31 eCollection Date: 2022-11-01 DOI:10.1007/s44164-022-00031-y
Harvinder Singh Virk, Ketul C Popat
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Abstract

Titanium and its alloys are used to make different medical devices such as stents, artificial heart valves, and catheters for cardiovascular diseases due to their superior biocompatibility. Thrombus formation begins on the surface of these devices as soon as they encounter blood. This leads to the formation of blood clots, which obstructs the flow of blood that leads to severe complications. Recent advancements in nanoscale fabrication and superhydrophobic surface modification techniques have demonstrated that these surfaces have antiadhesive properties and the ability to reduce thrombosis. In this study, the interaction of erythrocytes and whole blood clotting kinetics on superhydrophobic titanium nanostructured surfaces was investigated. These surfaces were characterized for their wettability (contact angle), surface morphology and topography (scanning electron microscopy (SEM)), and crystallinity (glancing angled X-ray diffraction (GAXRD)). Erythrocyte morphology on different surfaces was characterized using SEM, and overall cell viability was demonstrated through fluorescence microscopy. The hemocompatibility of these surfaces was characterized using commercially available assays: thrombin generation assay thrombin generation, hemolytic assay hemolysis, and complement convertase assay complement activity. The results indicate that superhydrophobic titanium nanostructured surfaces had lower erythrocyte adhesion, less morphological changes in adhered cells, lower thrombin generation, lower complement activation, and were less cytotoxic compared to control surfaces. Thus, superhydrophobic titanium nanostructured surfaces may be a promising approach to prevent thrombosis for several medical devices.

红细胞与钛纳米结构表面的相互作用。
钛及其合金因其优越的生物相容性,被用于制造各种医疗器械,如支架、人工心脏瓣膜、心血管疾病导管等。一旦这些装置遇到血液,它们的表面就会形成血栓。这会导致血栓的形成,从而阻碍血液的流动,从而导致严重的并发症。纳米级制造和超疏水表面修饰技术的最新进展表明,这些表面具有抗粘附性能和减少血栓形成的能力。在这项研究中,研究了红细胞和全血在超疏水钛纳米结构表面的相互作用。对这些表面进行了润湿性(接触角)、表面形貌和形貌(扫描电子显微镜(SEM))和结晶度(掠角x射线衍射(GAXRD))表征。用扫描电镜观察不同表面的红细胞形态,并用荧光显微镜观察细胞的整体活力。这些表面的血液相容性使用市售的测定方法进行表征:凝血酶生成测定、凝血酶生成测定、溶血测定和补体转化酶测定补体活性。结果表明,与对照表面相比,超疏水钛纳米结构表面具有较低的红细胞粘附性,粘附细胞的形态变化较小,凝血酶生成较低,补体活化较低,细胞毒性较小。因此,超疏水钛纳米结构表面可能是一种很有前途的方法,以防止血栓形成的几种医疗设备。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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