The expression of POMC and AgRP in brain and kidney tissues at different stages of diabetic nephropathy rats

Diabetic nephropathy : DN Pub Date : 2021-01-01 DOI:10.2478/dine-2021-0008

Shasha Liu, J. Da, Jiayu Li, R. Dong, J. Yuan, Y. Zha

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引用次数: 1

Abstract

Abstract Objective To explore the changes of proopiomelanocortin (POMC) and Agouti-Related Peptide (AgRP) expression in brain and kidney tissues under insulin intervention at different stages of diabetic nephropathy (DN) rats. Methods The male Sprague-Dawley (SD) rats of DN were treated with high-fat diet for 8 weeks and induced by intraperitoneally injection of streptozotocin (30 mg/kg) for one time. Then DN rats were also injected insulin subcutaneously at 2–5 U/(kg·24 h) from initiation of the streptozotocin. Kidney tissue, blood sample, and 24 h-urine were collected to detect the ratio of kidney/body weight, blood glucose and 24-h urinary albumin excretion rate at different stages (4, 8, 12, and 16 weeks). Immunohistochemistry assay was used to measure the expression of POMC and AgRP at different stages of DN rats. Results The DN rats were established successfully. With the progression of DN, blood glucose, 24-h urinary albumin excretion rate and kidney body weight ratio increased significantly, while decreased when insulin was injected. Immunohistochemistry showed that the expression levels of POMC were decreased gradually in brain and kidney tissues. Conversely, the expression of AgRP in kidney was highest at week 8 and then decreased gradually. The effect of insulin on normalizing POMC and AgRP expression in brain and renal tissues was also observed in DKD rats. Conclusion With the progression of DN, the expression of POMC and AgRP in kidney tissues was observed at different stages of disease, and their expressions were significantly normalized by insulin. The mechanism of in situ expression of POMC and AGRP in kidney to the progression of DN needs further investigations.

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糖尿病肾病不同阶段大鼠脑、肾组织中POMC和AgRP的表达

【摘要】目的探讨胰岛素干预下糖尿病肾病(DN)大鼠不同阶段脑组织和肾脏组织中proopiomelanocortin (POMC)和agouti相关肽(AgRP)表达的变化。方法采用高脂饮食治疗DN雄性SD大鼠8周，腹腔注射链脲佐菌素(30 mg/kg) 1次。链脲佐菌素给药后皮下注射胰岛素2 ~ 5 U/(kg·24 h)。取肾组织、血、24 h尿，检测各组不同阶段(4、8、12、16周)肾/体重、血糖及24 h尿白蛋白排泄率。采用免疫组化法检测DN大鼠不同时期POMC和AgRP的表达。结果成功建立DN大鼠。随着DN的进展，血糖、24小时尿白蛋白排泄率和肾重比均显著升高，而注射胰岛素则降低。免疫组化显示POMC在脑组织和肾组织中的表达水平逐渐降低。相反，AgRP在肾脏中的表达在第8周最高，随后逐渐下降。我们还观察了胰岛素对DKD大鼠脑和肾组织POMC和AgRP表达的影响。结论随着DN的进展，不同疾病阶段肾脏组织中POMC和AgRP的表达均有变化，且胰岛素使其表达明显正常化。POMC和AGRP在肾脏中原位表达对DN进展的影响机制有待进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Diabetic nephropathy : DN

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