IDENTIFIKASI SENYAWA BIOAKTIF EKSTRAK ETANOL BUAH PAKOBA MERAH (Syzygium sp.) SEBAGAI ANTIDIABETES DENGAN METODE TES TOLERANSI GLUKOSA PERORAL

IF 1 4区化学 Q4 CHEMISTRY, MULTIDISCIPLINARY

化学进展 Pub Date : 2021-11-30 DOI:10.35799/cp.14.2.2021.37175

Ahlan Sangkal

{"title":"IDENTIFIKASI SENYAWA BIOAKTIF EKSTRAK ETANOL BUAH PAKOBA MERAH (Syzygium sp.) SEBAGAI ANTIDIABETES DENGAN METODE TES TOLERANSI GLUKOSA PERORAL","authors":"Ahlan Sangkal","doi":"10.35799/cp.14.2.2021.37175","DOIUrl":null,"url":null,"abstract":"Buah pakoba merah memiliki kandungan senyawa bioaktif yang dapat dimanfaatkan sebagai antidiabetes. Penelitian ini bertujuan untuk mengidentifikasi senyawa bioaktif ekstrak etanol buah pakoba merah serta uji antidiabetes ekstrak dan fraksi-fraksinya. Identifikasi senyawa bioaktif dilakukan dengan cara maserasi dan pengujian metabolit sekunder serta pemantauan pola pemisahan senyawa dengan kromatografi lapis tipis (KLT). Pengujian antidiabetes dilakukan melalui uji in vivo dengan metode tes toleransi glukosa peroral (TTGO) pada kelompok hewan uji. Hasil identifikasi menunjukkan senyawa bioaktif buah pakoba merah mengandung senyawa alkaloid, terpenoid, steroid, flavonoid, saponin dan tanin. Hasil pemantauan pola pemisahan senyawa menunjukkan pola pemisahan yang hampir sama antara Ekstrak Etanol, Fraksi n-heksan, Fraksi EtOAc dan Fraksi n-Butanol sedangkan Fraksi Air menunjukkan hampir tidak terbentuk pola pemisahan. Hasil uji antidiabetes menunjukkan bahwa ekstrak EtOH, fraksi n-heksana, fraksi EtOAc, fraksi BuOH dan fraksi H2O dengan pembanding glibenklamid memiliki kemampuan sebagai antidiabetes ditinjau dari jumlah selisih penurunan kadar glukosa darah pada kelompok hewan uji dari T30 sampai T150. Penurunan rata-rata kadar glukosa darah kelompok kontrol sebesar 55,67 mg/dL, kelompok GB 116,66 mg/dL, kelompok ekstrak EtOH 100 mg/dL, kelompok fraksi n-heksana 100 mg/dL, kelompok fraksi EtOAc 110 mg/dL, kelompok fraksi BuOH 102,67 mg/dL dan kelompok fraksi H2O 94,33 mg/dL.ABSTRACT Pakoba merah fruit contains bioactive compounds that can be used as antidiabetic. This purpose of the research to identify bioactive compounds of the ethanol extract of pakoba merah fruit as well antidiabetic test of the extract and their fractions. Identification of bioactive compounds was carried out by maceration and secondary metabolite testing as well monitoring pattern separation of compounds using thin layer chromatography (TLC). Antidiabetic testing was carried out through an in vivo test using the oral test tolerance glucose (TTGO) method in the test animal group. The identification results showed that the bioactive compounds of pakoba merah fruit contained alkaloid, terpenoid, steroid, flavonoid, saponin and tannin. The results of monitoring pattern separation of compounds show that separation pattern was almost same between Ethanol Extract, n-hexane Fraction, EtOAc Fraction and n-Butanol Fraction while the H2O Fraction showed almost no separation pattern.The results of the antidiabetic test showed that the EtOH extract, n-hexane fraction, EtOAc fraction, BuOH fraction and H2O fraction with comparisons of glibenclamide had the ability to act as antidiabetic in terms of the difference in the decrease in blood glucose levels in the test animal groups from T30 to T150. The average decrease in blood glucose levels in the control group was 55.67 mg/dL, the GB group was 116.66 mg/dL, EtOH extract group was 100 mg/dL, the n-hexane fraction group was 100 mg/dL, EtOAc fraction group was 110 mg/dL, BuOH fraction group 102.67 mg/dL and H2O fraction group 94.33 mg/dL.","PeriodicalId":49662,"journal":{"name":"化学进展","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"化学进展","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.35799/cp.14.2.2021.37175","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 1

Abstract

Buah pakoba merah memiliki kandungan senyawa bioaktif yang dapat dimanfaatkan sebagai antidiabetes. Penelitian ini bertujuan untuk mengidentifikasi senyawa bioaktif ekstrak etanol buah pakoba merah serta uji antidiabetes ekstrak dan fraksi-fraksinya. Identifikasi senyawa bioaktif dilakukan dengan cara maserasi dan pengujian metabolit sekunder serta pemantauan pola pemisahan senyawa dengan kromatografi lapis tipis (KLT). Pengujian antidiabetes dilakukan melalui uji in vivo dengan metode tes toleransi glukosa peroral (TTGO) pada kelompok hewan uji. Hasil identifikasi menunjukkan senyawa bioaktif buah pakoba merah mengandung senyawa alkaloid, terpenoid, steroid, flavonoid, saponin dan tanin. Hasil pemantauan pola pemisahan senyawa menunjukkan pola pemisahan yang hampir sama antara Ekstrak Etanol, Fraksi n-heksan, Fraksi EtOAc dan Fraksi n-Butanol sedangkan Fraksi Air menunjukkan hampir tidak terbentuk pola pemisahan. Hasil uji antidiabetes menunjukkan bahwa ekstrak EtOH, fraksi n-heksana, fraksi EtOAc, fraksi BuOH dan fraksi H2O dengan pembanding glibenklamid memiliki kemampuan sebagai antidiabetes ditinjau dari jumlah selisih penurunan kadar glukosa darah pada kelompok hewan uji dari T30 sampai T150. Penurunan rata-rata kadar glukosa darah kelompok kontrol sebesar 55,67 mg/dL, kelompok GB 116,66 mg/dL, kelompok ekstrak EtOH 100 mg/dL, kelompok fraksi n-heksana 100 mg/dL, kelompok fraksi EtOAc 110 mg/dL, kelompok fraksi BuOH 102,67 mg/dL dan kelompok fraksi H2O 94,33 mg/dL.ABSTRACT Pakoba merah fruit contains bioactive compounds that can be used as antidiabetic. This purpose of the research to identify bioactive compounds of the ethanol extract of pakoba merah fruit as well antidiabetic test of the extract and their fractions. Identification of bioactive compounds was carried out by maceration and secondary metabolite testing as well monitoring pattern separation of compounds using thin layer chromatography (TLC). Antidiabetic testing was carried out through an in vivo test using the oral test tolerance glucose (TTGO) method in the test animal group. The identification results showed that the bioactive compounds of pakoba merah fruit contained alkaloid, terpenoid, steroid, flavonoid, saponin and tannin. The results of monitoring pattern separation of compounds show that separation pattern was almost same between Ethanol Extract, n-hexane Fraction, EtOAc Fraction and n-Butanol Fraction while the H2O Fraction showed almost no separation pattern.The results of the antidiabetic test showed that the EtOH extract, n-hexane fraction, EtOAc fraction, BuOH fraction and H2O fraction with comparisons of glibenclamide had the ability to act as antidiabetic in terms of the difference in the decrease in blood glucose levels in the test animal groups from T30 to T150. The average decrease in blood glucose levels in the control group was 55.67 mg/dL, the GB group was 116.66 mg/dL, EtOH extract group was 100 mg/dL, the n-hexane fraction group was 100 mg/dL, EtOAc fraction group was 110 mg/dL, BuOH fraction group 102.67 mg/dL and H2O fraction group 94.33 mg/dL.

查看原文本刊更多论文

鉴定红蕨果乙醇提取物(Syzygium sp)的生物活性化合物，以研究口服葡萄糖耐受性测试的抗糖尿病方法

红蕨果含有一种可用于抗糖尿病的生物活性化合物。这项研究的目的是确定红蕨果乙醇活性提取的物质以及它的抗糖尿病提取物和成分。生物活性化合物识别是通过体外受精和次级代谢测试以及监测薄层色谱分析的化合物分离模式进行的。抗糖尿病测试是通过活检进行的，通过动物群的葡萄糖耐受性测试进行的。鉴定显示，红蕨属植物的生物活性化合物中含有生物碱、松节油、类固醇、类黄酮、黄素和单宁。对化合物分离模式的监测显示，乙醇提取物、n-heksan馏分、EtOAc馏分和n-Butanol馏分之间几乎相同的分离模式，而水的馏分则表明分离模式几乎没有形成。反糖尿病检测结果表明，EtOH提取物、n-heksana的微量成分、EtOAc的微量成分、BuOH的微量成分和H2O的微量成分具有反糖尿病的能力，这取决于糖原体动物群中T30至T150的葡萄糖水平下降的几率。控制组血糖平均下降55.67 mg/dL, GB 116.66 mg/dL提品100 mg/dL, EtOAc 100 mg/dL, EtOAc 110 mg/dL, EtOAc 110 mg/dL, ntoac小组102.67 mg/dL, ntoac小组102.67 mg/dL, H2O 94.33 mg/dL。不带红蕨水果，一种可以用作抗糖尿病药物的生物活性化合物。这项研究的目的是鉴定红棕榈果的生物活性成分，并进行排毒测试。用更微妙的色谱学(TLC)来确定生物活性组合的识别。抗糖尿病测试用口服糖耐药性测试(ttkose)的方法在动物测试组中进行。标识再现表明，一种生物活性化合物，一种含有生物钙、松节油、类固醇、黄酮、黄斑和黄斑。商业监督的结果表明，不同的模式在乙醇Extract, n hexane Fraction, EtOAc Fraction和n-Butanol Fraction之间几乎是一样的，而H2O帧显示几乎没有分离模式。results》《EtOH extract antidiabetic测试那里那个,n-hexane fraction, EtOAc fraction, BuOH fraction和H2O和comparisons fraction of glibenclamide有美国不在乎to act antidiabetic》条款的《画》和《decrease in blood glucose水平测试中的小动物集团从T30到T150。控制小组的平均震级为55.67 mg/dL, GB组为116.66 mg/dL, EtOH extract group为100 mg/dL, EtOAc fraction group为110 mg/dL, EtOAc fraction group为10mg /dL, EtOAc fraction group为10mg /dL, EtOAc fraction group为10mg /dL, EtOAc fraction group为10mg /dL。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

化学进展化学-化学综合

CiteScore

2.10

自引率

15.40%

发文量

4325

审稿时长

2.5 months

期刊介绍： Progress in Chemistry is a peer-reviewed monthly journal. It is sponsored by the Chinese Academy of Sciences(CAS) and the National Natural Science Foundation of China(NSFC). It provides a forum to publish review papers of specialized topics covering the full spectrum of chemistry in Chinese or English, with emphasis on those topics of emerging research area. The reviews provide comprehensive information, including recent advances, development trends, as well as critical assessments about the subject. The readers interested are : researchers and students in chemistry and related areas, and policy decision-makers. Most members of the editorial board are famous scientists.