VALIDATION OF UV-VIS SPECTROPHOTOMETRIC METHOD TO DETERMINE DRUG RELEASE OF QUERCETIN LOADED-NANOEMULSION

Abstract

A simple validated UV/Vis spectrophotometric method to analyze quercetin released from the nanoemulsion matrix has been developed to study the drug release profile of quercetin loaded-nanoemulsion. Quercetin analysis was carried out at a maximum wavelength of 254 nm. The method showed linearity with a correlation coefficient (r) value of 0.9998 in the range of 4 - 12 μg/mL. The results also demonstrated that the procedure is accurate and precise, with recovery (%) in the range of 99.65 - 100.1312 % and RSD (%) as ≤ 2%. The limit of detection (LOD) and limit of quantitation (LOQ) values were found to be 4.0535 μg/mL and 13.5118 μg/mL, respectively. The developed method was found to be valid to analyze quercetin released in nanoemulsion preparations.