Dose-dependent disruptive effects of melatonin, light and temperature on regeneration in the planarian Phagocata gracilis

IF 0.9 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Marine and Freshwater Behaviour and Physiology Pub Date : 2019-03-04 DOI:10.1080/10236244.2019.1640062

S. C. Beeching, Brian B. Merritt

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引用次数: 0

Abstract

ABSTRACT Many critical cellular processes, including cell proliferation and tissue repair, exhibit melatonin sensitivity. We examined the effects of exogenous melatonin, photoperiod and temperature on regeneration in Phagocata gracilis, a common North American turbellarian. Planarian regeneration involves the proliferation and migration of pluripotent stem cells capable of replacing missing body components. A characteristic mass of cells (blastema) forms during planarian regeneration, and its formation, growth and differentiation provide reliable endpoints for the study of regenerative processes. We tested the effects of exogenous melatonin at two concentrations and three photoperiods on both head and tail regenerates under seasonal diurnal temperature regimes. High temperatures and melatonin treatment increased mortality in P. gracilis, and surviving melatonin-treated subjects exhibited delayed regeneration. Asymmetrical and deficient regeneration was more common in melatonin-treated, high temperature and dark treated subjects. Additionally, high temperature and melatonin induced a novel locomotor dysfunction.

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褪黑素、光和温度对涡虫薄壁吞噬虫再生的剂量依赖性破坏作用

许多关键的细胞过程，包括细胞增殖和组织修复，都表现出褪黑素敏感性。我们研究了外源褪黑素、光周期和温度对北美常见的涡虫——薄壁吞噬虫再生的影响。涡虫再生涉及能够替代缺失身体成分的多能干细胞的增殖和迁移。在涡虫再生过程中，会形成大量的细胞(胚基)，它的形成、生长和分化为研究再生过程提供了可靠的终点。我们测试了外源褪黑素在两种浓度和三个光周期下对季节性昼夜温度下头部和尾部再生的影响。高温和褪黑激素治疗增加了薄叶草的死亡率，存活的褪黑激素治疗对象表现出再生延迟。不对称和再生缺陷在褪黑激素处理、高温和黑暗处理的受试者中更为常见。此外，高温和褪黑激素诱导了一种新的运动功能障碍。

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来源期刊

Marine and Freshwater Behaviour and Physiology 生物-海洋与淡水生物学

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Marine and Freshwater Behaviour and Physiology is devoted to the publication of papers covering field and laboratory research into all aspects of the behaviour and physiology of all marine and freshwater animals within the contexts of ecology, evolution and conservation. As the living resources of the world’s oceans, rivers and lakes are attracting increasing attention as food sources for humans and for their role in global ecology, the journal will also publish the results of research in the areas of fisheries biology and technology where the behaviour and physiology described have clear links to the contexts mentioned above. The journal will accept for publication Research Articles, Reviews, Rapid Communications and Technical Notes (see Instructions for authors for details). In addition, Editorials, Opinions and Book Reviews (invited and suggested) will also occasionally be published. Suggestions to the Editor-In-Chief for Special Issues are encouraged and will be considered on an ad hoc basis. With the goal of supporting early career researchers, the journal particularly invites submissions from graduate students and post-doctoral researchers. In addition to recognising the time constraints and logistical limitations their research often faces, and their particular need for a prompt review process, accepted articles by such researchers will be given prominence within the journal (see Instructions for authors for details).