Morphological manifestations of the dynamics of catecholamines binding by erythrocytes during activation and blockade of adrenergic regulatory mechanisms

Abstract

Objective - to study, the dynamics of catecholamine binding on erythrocytes when modeling the stimulation and blockade of adrenergic regulation mechanisms using the cytological method Material and methods. The number of catecholamine granules on erythrocytes was determined using silver nitrate impregnation under conditions of administration of anapriline -adrenergic receptor blocker (2 mg/kg), acute stress, activation of noradrenergic systems (maprotiline, 10 mg/kg) and a combination of these effects. Results. The number of catecholamine granules in intact animals is 145-155 pieces/40 erythrocytes. Medium-sized granules are more common (0.6-0.9 m). After the administration of a -adrenergic receptor blocker, the total number of catecholamine granules decreases 2.8 times due to large and medium-sized granules. Under conditions of acute stress, the total number of granules increases almost 2 times due to small granules, which may be a sign of sensitization of erythrocyte membranes to catecholamines. Stimulation of the noradrenergic system causes a 20% decrease in the number of catecholamine granules due to a decrease in the number of small and medium sized granules. Under stress against the background of activation of the noradrenergic system, the number of granules on erythrocytes is reduced, which may be a sign of adrenergic receptor desensitization. Conclusions. The number of catecholamine granules on erythrocytes decreases after the administration of a -adrenergic receptor blocker and increases during acute stress. Stimulation of the noradrenergic system is accompanied by a decrease in the binding of catecholamines, especially under conditions of acute stress, which indicates desensitization of erythrocyte adrenergic receptors. The cytological method is sensitive enough to observe the reception of catecholamines by erythrocytes when exposed to adrenergic structures.