Gene expression profile of MAP kinase PTC1 mutant exposed to Aflatoxin B1: dysfunctions of gene expression in glucose utilization and sphingolipid metabolism

Abstract

Aflatoxin B1 (AFB1) is a harmful and cancer-causing mycotoxin generated by Aspergillus flavus. Its mechanism of toxicity has not been fully clarified and further research is required. In this study, we attempted to further clarify aflatoxin B1 toxicity using the results of S. cerevisiae gene expression analysis. In a Ser/Thr phosphatase 2C disruptant (ptc1Δ) with weakened activity of anti-toxic components (cell wall and membrane), the addition of low concentrations of sodium dodecyl sulfate resulted in elevated susceptibility to AFB1. From the microarray results, expression changes in DNA synthesis or repair, sphingolipid metabolism, glucose metabolism, and cell wall-related genes were well detected. Our results indicate that AFB1 causes sphingolipid metabolism disorder, leading to dysfunction in signal secretion and inhibition of efficient glucose metabolism, which supplies the materials for cell wall proteins and cellular components, resulting in repression of the stress response to external toxicants.