Standardization of sterilization protocol for explants and its suitability for direct organogenesis in tuberose cv. Arka Vaibhav

IF 0.2 Q4 HORTICULTURE
Mahananda Patil, T. Bharathi, T. Usharani, Rajiv Kumar, B. S. Kulkarni
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Abstract

A study was carried out to standardize the sterilization protocol for different explants (terminal stem scale,immature flower bud and tepal segment) and to select the suitable explant for the direct organogenesis of tuberose cv. Arka Vaibhav. The highest survival per cent (100) and uncontaminated cultures (0.00) of terminal stem scale explant was observed in pre-treatment with overnight soaking of terminal stem scale in the solution comprising carbendazim (0.1%), chlorothalonil (0.05%) and myristyl trimethyl ammonium bromide (cetrimide) (0.05%) and subsequently surface sterilization with 70% ethanol (1 min), 4% sodium hypochlorite (10 min) followed by 0.1% HgCl2 (15 min). The explant immature flower bud recorded the highest survival per cent (100) and maximum aseptic cultures in the treatment T1 comprised of 1.0 drop Tween-20 + 70% ethanol (30 sec) and 1% sodium hypochlorite (3 min). Pre-treatment of tepal segment explant in 0.1% carbendazim (30 min) solution followed by surface sterilization with combination of 1.0 drop Tween-20 + 70% ethanol (30 sec) followed by 1% sodium hypochlorite (3 min) registered 91.66% of survival with the minimum contamination (10%) in the treatment. Among the three explants used, the terminal stem scale was found suitable for direct organogenesis with early greenness (5.72 days) and highly responsive to shoot induction (100%) in MS medium supplemented with 4 mg/L BAP + 0.1mg/L IAA. Other two explants viz., immature flower bud and tepal segment failed to respond for direct organogenesis by shoot induction instead produced profuse callus.
外植体灭菌规程的标准化及其在薯类植物直接器官发生中的适用性。Arka Vaibhav
为规范不同外植体(顶茎鳞片、未成熟花芽和花被片)的灭菌方案,选择适合块茎直接器官发生的外植体进行了研究。Arka Vaibhav。用多菌灵(0.1%)、百菌清(0.05%)、百菌清(0.05%)三甲基溴化肉豆醇铵(0.05%)溶液浸泡根茎鳞片,预处理后用70%乙醇(1 min)、4%次氯酸钠(10 min)、0.1%盐酸(15 min)表面杀菌,根茎鳞片外植体存活率最高(100),无污染培养物最高(0.00)。在1.0滴Tween-20 + 70%乙醇(30秒)和1%次氯酸钠(3分钟)的处理T1下,外植体未成熟花芽的存活率最高(100),无菌培养量最大。花被片外植体在0.1%多菌肼溶液中预处理(30 min),然后用1.0滴Tween-20 + 70%乙醇(30秒)和1%次氯酸钠(3 min)联合杀菌,存活率为91.66%,处理中污染最小(10%)。3个外植体中,顶茎鳞片在添加4mg /L BAP + 0.1mg/L IAA的MS培养基中,绿度较早(5.72 d),对茎部诱导反应高(100%),适合直接器官发生。另外两个外植体即未成熟花芽和花被片没有直接诱导器官发生的反应,而是产生了大量的愈伤组织。
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来源期刊
Journal of Horticultural Sciences
Journal of Horticultural Sciences Agricultural and Biological Sciences-Plant Science
CiteScore
0.30
自引率
0.00%
发文量
0
审稿时长
6 weeks
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