The effect of pectinase pretreatment on the extraction of essential oil from the seed kernels of Nutmeg (Myristica fragrans)

Q4 Agricultural and Biological Sciences

Annals of Agri Bio Research Pub Date : 2022-07-09 DOI:10.4314/br.v20i2.9

W. Iko, K. Omeje, V. Ozougwu, S. Eze, F. C. Chilaka

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引用次数: 0

Abstract

In this study, the seed kernels of Nutmeg (Myristica fragrans) were pretreated with partially purified pectinase (PPP) prior to hydrodistillation to enhance the extraction of essential oil. The yield of the essential oil obtained after pretreatment with PPP was 4.70%. GC-MS analysis of the essential oils obtained after pretreatments with PPP showed a total of 33 constituents classified into monoterpenes (51.52%), sesquiterpenes (21.21%) and phenylpropanoids/aromatic (27.27%) compounds. Comparison was made by pretreating the nutmeg seeds with a pure pectinase (CP) obtained from Sigma-Aldrich. The fraction obtained when pretreated with CP was made up of 32 components with monoterpenes (51.31%), sesquiterpenes (18.75%) and phenylpropanoids/aromatic (28.12%) compounds. The major components of essential oils obtained after pretreatments with PPP and CP were sabinene, α-pinene, β-pinene and myristicin. This research shows that the extraction of Myristica fragrans seed kernel essential oil can be improved using PPP when compared with the yield of the control (3.80%) obtained from the seed kernels that were not pretreated with the enzymes prior to hydrodistillation.

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果胶酶预处理对肉豆蔻籽仁精油提取的影响

本研究采用部分纯化果胶酶(PPP)对肉豆蔻(Myristica fragrans)种子仁进行预处理，然后进行加氢蒸馏，以提高其精油的提取率。经PPP预处理得到的精油得率为4.70%。经PPP预处理后得到的精油的GC-MS分析显示，共有33种成分可分为单萜(51.52%)、倍半萜(21.21%)和苯丙素/芳香族(27.27%)化合物。用Sigma-Aldrich公司的纯果胶酶(CP)预处理肉豆蔻种子进行比较。经CP预处理后得到的馏分由单萜(51.31%)、倍半萜(18.75%)和苯基丙烷/芳香族(28.12%)化合物组成的32种组分组成。PPP和CP预处理后得到的精油主要成分为sabinene、α-蒎烯、β-蒎烯和肉豆素。本研究表明，与未加水蒸馏预处理的肉豆蔻籽仁精油的提取率(3.80%)相比，PPP可提高肉豆蔻籽仁精油的提取率。

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来源期刊

Annals of Agri Bio Research Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

0.30

自引率

0.00%

发文量

期刊介绍： An international peer reviewed semi-annual journal,publishing original research papers and critical mini-reviews in basic and applied aspects of agricultural and biological sciences.