A.R. Nayak , J.S. Nair , M.V. Hegde , P.K. Ranjekar , U. Pant
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引用次数: 8
两种蚊子的基因组分析
白纹伊蚊和双纹库蚊DNA的Tm分别为78和79°C,G-C含量分别为21.2和23.6%。这两种物质的高分辨率热变性(HRTD)分析显示,在富含GC的序列中,碱基组成的不均匀性更大。高度重复的DNA序列占基因组的8-10%,动力学复杂度为5.1×105 np。在Cot 0.1 M.S.从未屏蔽的DNA(>;20 kbp)中分离出的S1核酸酶抗性重复双链体显示出200–500 bp的重复类别。两种蚊子的限制性内切酶切割分析显示存在具有MboI和Sau3AI的重复序列家族。用甲基化特异性限制性内切酶消化这两个物种的DNA表明甲基化是广泛的,并且不限于CpG二核苷酸单独。除了HRTD分析外,两个物种之间有非常显著的相似性。
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