Antigenotoxic potential of curcumin and carvacrol against malathion-induced DNA damage in cultured human peripheral blood and its relation to GSTM1 and GSTT1 polymorphism

Biomarkers and Genomic Medicine Pub Date : 2015-09-01 DOI:10.1016/j.bgm.2015.02.002

Neeraj Kumar , Anita Yadav , Sachin Gulati , Kanupriya , Neeraj Aggarwal , Ranjan Gupta

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引用次数: 6

Abstract

The aerial application of malathion, a widely used organophosphate insecticide, has raised public concerns about its potential adverse health effects. We, therefore, studied the antigenotoxic potential of curcumin and carvacrol against malathion-induced DNA damage using sister chromatid exchange (SCE) as a biomarker of genotoxicity. To observe the antigenotoxic potential of curcumin and carvacrol, heparinized fresh blood from healthy individuals was treated with 30 μg/mL of malathion in the presence of curcumin and carvacrol. Curcumin at concentrations of 25 μg/mL and 50 μg/mL had significantly reduced (p < 0.05) the frequency of SCE as compared to malathion-exposed sample. Similarly, carvacrol showed significant (p < 0.05) antigenotoxic effect at concentrations of 2.5 μg/mL and 5.0 μg/mL against malathion. We also studied the effect of GSTT1 and GSTM1 on the genotoxicity of malathion and antigenotoxic potential of curcumin and carvacrol. We observed that there is a statistically significant (p < 0.05) reduction in the frequency of SCE in case of curcumin and carvacrol as compared to malathion, but we did not observe any significant relationship (p > 0.05) between GSTT1 and GSTM1 polymorphism and the genotoxicity of malathion and antigenotoxic potential of curcumin and carvacrol.

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姜黄素和香芹酚对马拉硫磷诱导的人外周血DNA损伤的抗遗传毒性及其与GSTM1和GSTT1多态性的关系

马拉硫磷是一种广泛使用的有机磷杀虫剂，其空中施用引起了公众对其潜在健康不良影响的担忧。因此，我们利用姐妹染色单体交换（SCE）作为遗传毒性的生物标志物，研究了姜黄素和香芹酚对马拉硫磷诱导的DNA损伤的抗原性潜力。为了观察姜黄素和香芹酚的抗原性，在姜黄素和香樟酚存在的情况下，用30μg/mL马拉硫磷处理来自健康个体的肝素化新鲜血液。与马拉硫磷暴露样品相比，浓度为25μg/mL和50μg/mL的姜黄素显著降低了SCE的频率（p＜0.05）。同样，香芹酚在2.5μg/mL和5.0μg/mL浓度下对马拉硫磷表现出显著的（p＜0.05）抗原毒性作用。我们还研究了GSTT1和GSTM1对马拉硫磷的遗传毒性以及姜黄素和香芹酚的抗原性的影响。我们观察到，与马拉硫磷相比，姜黄素和香芹酚的SCE频率在统计学上显著降低（p＜0.05），但我们没有观察到GSTT1和GSTM1多态性与马拉硫磷的遗传毒性和姜黄素和香樟酚的抗原毒性潜力之间的任何显著关系（p＞0.05）。

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Biomarkers and Genomic Medicine

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