Involvement of polyamines in epidermal growth factor (EGF), transforming growth factor (TGF)-α and -β1 action on culture of L6 and fetal bovine myoblasts

International Journal of Biochemistry Pub Date : 1994-07-01 DOI:10.1016/0020-711X(94)90082-5

S. Blachowski , T. Motyl , Katarzyna Grzelkowska , Maria Kasterka , A. Orzechowski , Boźena Interewicz

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引用次数: 21

Abstract

1.
1. α-Difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase significantly abolished stimulation of protein synthesis evoked by EGF, TGF-α or -β 1 in L6 and fetal bovine myoblasts.
2.
2. The participation of polyamines in early events evoked by growth factors was shown by a significant stimulation of ornithine decarboxylase and Sdenosylmethionine decarboxylase activity as well as increased concentration of spermidine and spermine in L6 cells exposed to TGF-α and EGF.
3.
3. TGF-β 1 at a high concentration (1 ng/ml) increased protein synthesis in L6 myoblasts but inhibited it in fetal bovine myoblasts. Metabolic effects of TGF-β 1 in L6 cells was associated with an enhancement of decarboxylase activities, however there were no significant changes in cellular polyamine concentrations. Presented data suggest that polyamines are involved in the signal transduction pathway of EGF, TGF-α, and -β 1 in L6 and fetal bovine myoblasts.

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多胺参与表皮生长因子（EGF）、转化生长因子（TGF）-α和-β1对L6和胎牛成肌细胞培养的作用

1.1.α-二氟甲基鸟氨酸，一种不可逆的鸟氨酸脱羧酶抑制剂，显著消除了L6和胎牛成肌细胞中EGF、TGF-α或-β1引起的蛋白质合成刺激。在暴露于TGF-α和EGF的L6细胞中，多胺参与生长因子引起的早期事件表现为显著刺激鸟氨酸脱羧酶和缩氨酰甲硫氨酸脱羧酶活性，以及精脒和精胺浓度增加。3.3。高浓度（1ng/ml）的TGF-β1增加了L6成肌细胞中的蛋白质合成，但抑制了胎牛成肌细胞的蛋白质合成。转化生长因子-β1在L6细胞中的代谢作用与脱羧酶活性的增强有关，但细胞多胺浓度没有显著变化。现有数据表明，多胺参与L6和胎牛成肌细胞中EGF、TGF-α和-β1的信号转导途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Biochemistry

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