Effect of Selenium on Lung Injury Induced by Limb Ischemic Reperfusion Injury in Sprague-Dawley Rats.

IF 0.8 Q4 PERIPHERAL VASCULAR DISEASE

Vascular Specialist International Pub Date : 2023-11-10 DOI:10.5758/vsi.230065

Dudy Arman Hanafy, Christha Zenithy Tamburian, Lisnawati Rachmadi, Gerald Aldian Wijaya, Widya Trianita Suwatri, Sugisman

{"title":"Effect of Selenium on Lung Injury Induced by Limb Ischemic Reperfusion Injury in Sprague-Dawley Rats.","authors":"Dudy Arman Hanafy, Christha Zenithy Tamburian, Lisnawati Rachmadi, Gerald Aldian Wijaya, Widya Trianita Suwatri, Sugisman","doi":"10.5758/vsi.230065","DOIUrl":null,"url":null,"abstract":"Purpose: : Ischemia-reperfusion injury (IRI) plays an important role in the pathophysiology of acute limb ischemia, leading to damage to distant organs, including the lungs. A complex mechanism is involved in the formation of reactive oxygen species (ROS), release of inflammatory mediators, and neutrophil activation. One strategy to reduce the damage is administering selenium, an antioxidant enzyme component that can bind ROS and protect cells. This study aimed to compare the degree of lung injury due to limb IRI in Sprague-Dawley (SD) rats with selenium administration versus those without selenium treatment.Materials and methods: : Fifteen male SD rats were divided into three groups: the control group (Group A), the ischemia-reperfusion with pre-reperfusion selenium (Group B), and the ischemia-reperfusion with post-reperfusion selenium (Group C). All animals underwent two hours of limb ischemia and three hours of reperfusion. Selenium was given intravenously at a dose of 0.2 mg/kg body weight. After reperfusion, lung specimens were histopathologically examined.Results: : The median degree of lung injury was severe in Group A, mild in Group B, and moderate in Group C (P=0.01). Post hoc analysis revealed a significant difference in the degree of lung injury between Groups A and B (P=0.01), while a comparison between Groups A and C (P=0.06) and Groups B and C (P=0.31) revealed no significant difference.Conclusion: : The administration of pre-reperfusion selenium significantly decreases lung injury induced by limb ischemia-reperfusion in SD rats.","PeriodicalId":52311,"journal":{"name":"Vascular Specialist International","volume":"39 ","pages":"36"},"PeriodicalIF":0.8000,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636257/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vascular Specialist International","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5758/vsi.230065","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PERIPHERAL VASCULAR DISEASE","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: : Ischemia-reperfusion injury (IRI) plays an important role in the pathophysiology of acute limb ischemia, leading to damage to distant organs, including the lungs. A complex mechanism is involved in the formation of reactive oxygen species (ROS), release of inflammatory mediators, and neutrophil activation. One strategy to reduce the damage is administering selenium, an antioxidant enzyme component that can bind ROS and protect cells. This study aimed to compare the degree of lung injury due to limb IRI in Sprague-Dawley (SD) rats with selenium administration versus those without selenium treatment.

Materials and methods: : Fifteen male SD rats were divided into three groups: the control group (Group A), the ischemia-reperfusion with pre-reperfusion selenium (Group B), and the ischemia-reperfusion with post-reperfusion selenium (Group C). All animals underwent two hours of limb ischemia and three hours of reperfusion. Selenium was given intravenously at a dose of 0.2 mg/kg body weight. After reperfusion, lung specimens were histopathologically examined.

Results: : The median degree of lung injury was severe in Group A, mild in Group B, and moderate in Group C (P=0.01). Post hoc analysis revealed a significant difference in the degree of lung injury between Groups A and B (P=0.01), while a comparison between Groups A and C (P=0.06) and Groups B and C (P=0.31) revealed no significant difference.

Conclusion: : The administration of pre-reperfusion selenium significantly decreases lung injury induced by limb ischemia-reperfusion in SD rats.

查看原文本刊更多论文

硒对Sprague-Dawley大鼠肢体缺血再灌注损伤所致肺损伤的影响。

目的：缺血再灌注损伤（IRI）在急性肢体缺血的病理生理学中起着重要作用，导致包括肺部在内的远端器官损伤。一种复杂的机制涉及活性氧（ROS）的形成、炎症介质的释放和中性粒细胞的激活。减少损伤的一种策略是服用硒，硒是一种能够结合ROS并保护细胞的抗氧化酶成分。本研究旨在比较施硒与未施硒的Sprague-Dawley（SD）大鼠因肢体IRI引起的肺损伤程度。材料与方法：将15只雄性SD大鼠分为三组：对照组（A组）、再灌注前硒组（B组）和再灌注后硒组（C组）。所有动物均接受了两小时的肢体缺血和三小时的再灌注。硒是以0.2毫克/公斤体重的剂量静脉注射的。再灌注后，对肺标本进行组织病理学检查。结果：A组的中位肺损伤程度为严重，B组为轻度，C组为中度（P=0.01）。事后分析显示，A组和B组的肺损伤程度有显著差异（P=0.001），而A组和C组（P=0.06）与B组和C队（P=0.031）的比较无显著差异。结论：再灌注前硒能显著减轻SD大鼠肢体缺血再灌注所致的肺损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊