Vascular persistence following precision micropuncture

IF 1.9 4区医学 Q3 HEMATOLOGY

Microcirculation Pub Date : 2023-11-10 DOI:10.1111/micc.12835

Summer N. Horchler, Patrick C. Hancock, Mingjie Sun, Alexander T. Liu, Sameer Massand, Jessica C. El-Mallah, Dana Goldenberg, Olivia Waldron, Mary E. Landmesser, Shailaja Agrawal, Srinivas V. Koduru, Dino J. Ravnic

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引用次数: 0

Abstract

Objective

The success of engineered tissues continues to be limited by time to vascularization and perfusion. Recently, we described a simple microsurgical approach, termed micropuncture (MP), which could be used to rapidly vascularize an adjacently placed scaffold from the recipient macrovasculature. Here we studied the long-term persistence of the MP-induced microvasculature.

Methods

Segmental 60 μm diameter MPs were created in the recipient rat femoral artery and vein followed by coverage with a simple Type 1 collagen scaffold. The recipient vasculature and scaffold were then wrapped en bloc with a silicone sheet to isolate intrinsic vascularization. Scaffolds were harvested at 28 days post-implantation for detailed analysis, including using a novel artificial intelligence (AI) approach.

Results

MP scaffolds demonstrated a sustained increase of vascular density compared to internal non-MP control scaffolds (p < 0.05) secondary to increases in both vessel diameters (p < 0.05) and branch counts (p < 0.05). MP scaffolds also demonstrated statistically significant increases in red blood cell (RBC) perfused lumens.

Conclusions

This study further highlights that the intrinsic MP-induced vasculature continues to persist long-term. Its combination of rapid and stable angiogenesis represents a novel surgical platform for engineered scaffold and graft perfusion.

Abstract Image

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精密微穿刺后的血管持久性。

目的：工程组织的成功仍然受到血管化和灌注时间的限制。最近，我们描述了一种简单的显微外科方法，称为微穿刺（MP），可用于从受体大血管系统快速为相邻放置的支架提供血管。在这里，我们研究了MP诱导的微血管的长期持续性。方法：Segmental 60 在受体大鼠股动脉和静脉中产生直径为μm的MP，然后用简单的1型胶原支架覆盖。然后用硅胶片整体包裹受体血管系统和支架，以分离内在血管形成。脚手架于28日收割植入后几天进行详细分析，包括使用新型人工智能（AI）方法。结果：与内部非MP对照支架相比，MP支架显示出血管密度的持续增加（p 结论：这项研究进一步强调了MP诱导的固有血管系统继续长期存在。它结合了快速和稳定的血管生成，为工程支架和移植物灌注提供了一种新的手术平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microcirculation 医学-外周血管病

CiteScore

5.00

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.