Dried blood spot-based detection of serological profiles of hepatitis B and C infections and their prevalence in Cambodia

GastroHep Pub Date : 2021-06-02 DOI:10.1002/ygh2.468
Bunthen E, Ko Ko, Shintaro Nagashima, Serge Ouoba, Md Razeen Ashraf Hussain, Aya Sugiyama, Tomoyuki Akita, Masayuki Ohisa, Channarena Chuon, Bunsoth Mao, Md Shafiqul Hossain, Vichit Ork, Junko Tanaka
{"title":"Dried blood spot-based detection of serological profiles of hepatitis B and C infections and their prevalence in Cambodia","authors":"Bunthen E,&nbsp;Ko Ko,&nbsp;Shintaro Nagashima,&nbsp;Serge Ouoba,&nbsp;Md Razeen Ashraf Hussain,&nbsp;Aya Sugiyama,&nbsp;Tomoyuki Akita,&nbsp;Masayuki Ohisa,&nbsp;Channarena Chuon,&nbsp;Bunsoth Mao,&nbsp;Md Shafiqul Hossain,&nbsp;Vichit Ork,&nbsp;Junko Tanaka","doi":"10.1002/ygh2.468","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aims</h3>\n \n <p>This study aims to examine the diagnostic accuracy of dried blood spot (DBS) samples contrast to serum samples for detection of hepatitis B virus (HBV) and hepatitis C virus (HCV) sero-markers in large scale epidemiological study in the resource limited settings and then to determine the prevalence of each sero-marker from DBS samples collected during 2017 Cambodia nationwide study.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>This study included 921 paired DBS and serum samples. HBsAg, HBsAg-HQ, anti-HBs, anti-HBc and anti-HCV were detected in all DBS/serum pairs but HBeAg and anti-HBe in 109 DBS/serum pairs using chemiluminescent enzyme immunoassay (CLEIA). Thereafter, the individual DBS's diagnostic accuracy was calculated. Additionally, the prevalence of each sero-marker was calculated in 4541 DBS samples from nationwide study in Cambodia.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The sensitivity of DBS for detection of HBsAg, HBsAg-HQ, HBeAg and antiHBe were high (≥90%) with 100% specificity. Anti-HBc and anti-HBs showed low sensitivity (70.73% and 69.25% respectively) than other sero-markers, but their diagnostic accuracies were 94.58% and 89.32%. Therefore, the overall prevalence of HBsAg, HBsAg-HQ, anti-HBc, anti-HBs and anti-HCV using DBS samples in Cambodia were 2.38%, 2.53%, 14.27%, 31.84% and 0.15%, respectively. HBeAg and anti-HBe positivity among HBsAg positives were 31.48% and 53.7%.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Our study indicates that DBS has high diagnostic accuracy for HBsAg, HBsAg-HQ, HBeAg and anti-HBe. Having the benefit of simple procedure, easy and compact transportation and storage, it is considered as an alternative to serum samples in examining the serological profiles of HBV infection through a large-scale epidemiological study in the resource limited settings.</p>\n </section>\n </div>","PeriodicalId":12480,"journal":{"name":"GastroHep","volume":"3 4","pages":"247-253"},"PeriodicalIF":0.0000,"publicationDate":"2021-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ygh2.468","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"GastroHep","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/ygh2.468","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3

Abstract

Aims

This study aims to examine the diagnostic accuracy of dried blood spot (DBS) samples contrast to serum samples for detection of hepatitis B virus (HBV) and hepatitis C virus (HCV) sero-markers in large scale epidemiological study in the resource limited settings and then to determine the prevalence of each sero-marker from DBS samples collected during 2017 Cambodia nationwide study.

Methods

This study included 921 paired DBS and serum samples. HBsAg, HBsAg-HQ, anti-HBs, anti-HBc and anti-HCV were detected in all DBS/serum pairs but HBeAg and anti-HBe in 109 DBS/serum pairs using chemiluminescent enzyme immunoassay (CLEIA). Thereafter, the individual DBS's diagnostic accuracy was calculated. Additionally, the prevalence of each sero-marker was calculated in 4541 DBS samples from nationwide study in Cambodia.

Results

The sensitivity of DBS for detection of HBsAg, HBsAg-HQ, HBeAg and antiHBe were high (≥90%) with 100% specificity. Anti-HBc and anti-HBs showed low sensitivity (70.73% and 69.25% respectively) than other sero-markers, but their diagnostic accuracies were 94.58% and 89.32%. Therefore, the overall prevalence of HBsAg, HBsAg-HQ, anti-HBc, anti-HBs and anti-HCV using DBS samples in Cambodia were 2.38%, 2.53%, 14.27%, 31.84% and 0.15%, respectively. HBeAg and anti-HBe positivity among HBsAg positives were 31.48% and 53.7%.

Conclusion

Our study indicates that DBS has high diagnostic accuracy for HBsAg, HBsAg-HQ, HBeAg and anti-HBe. Having the benefit of simple procedure, easy and compact transportation and storage, it is considered as an alternative to serum samples in examining the serological profiles of HBV infection through a large-scale epidemiological study in the resource limited settings.

Abstract Image

基于干血点的乙型肝炎和丙型肝炎血清学特征检测及其在柬埔寨的流行情况
目的本研究旨在在资源有限的环境下,在大规模流行病学研究中,检验干血点(DBS)样本与血清样本对比检测乙型肝炎病毒(HBV)和丙型肝炎病毒(HCV)血清标志物的诊断准确性,然后确定2017年收集的干血点样本中每种血清标记物的流行率柬埔寨全国性研究。方法本研究包括921份配对DBS和血清样本。化学发光酶免疫测定法(CLEIA)在所有DBS/血清对中均检测到HBsAg、HBsAg-HQ、抗-HBs、抗-HBc和抗-HCV,但在109对DBS/血液对中检测到HBeAg和抗-HBe。然后,计算个体DBS的诊断准确性。此外,在柬埔寨全国研究的4541个DBS样本中计算了每种血清标志物的流行率。结果DBS检测HBsAg、HBsAg HQ、HBeAg和抗HBe的敏感性高(≥90%),特异性达100%。抗-HBc和抗-HBs的敏感性较低(分别为70.73%和69.25%),但其诊断准确率分别为94.58%和89.32%。HBsAg阳性者中HBeAg和抗-HBe阳性率分别为31.48%和53.7%。它具有程序简单、运输和储存方便紧凑的优点,被认为是在资源有限的环境中通过大规模流行病学研究来检测HBV感染血清学特征的血清样本的替代品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信