Rapid Simultaneous Determination of Cyanide, Azide, and Ethanol in Whole Blood Using Headspace Gas Chromatography-Mass Spectrometry

IF 1.2 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2023-10-27 DOI:10.1007/s10337-023-04289-6

Takaya Murakami, Yoshiaki Iwamuro, Yuki Sakamoto, Eriko Minami, Reiko Ishimaru, Hitoshi Tsuchihashi, Satoshi Chinaka

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Abstract

The rapid detection and quantification of toxic substances and alcohol in biological specimens stand as imperative tasks, particularly in instances of unnatural demise and poisoning incidents. In this study, we developed a high-throughput simultaneous method for analyzing cyanide, azide, and ethanol in whole blood. This methodology involves a simple manual pretreatment step, entailing the sequential addition of an internal standard, ascorbic acid, and phosphoric acid to whole blood samples (within 1 min), followed by headspace gas chromatography-mass spectrometry analysis (within 14 min). The method exhibits high linearity (correlation coefficients, > 0.9996), sensitivities of ng/mL-level detection, and satisfactory precision (relative standard deviation of less than 5.8% for cyanide, 8.8% for azide, and 2.9% for ethanol) and trueness (relative error within 7.3% for cyanide, 17% for azide, and 6.9% for ethanol). The proposed method, which does not require complex analytical techniques and possesses several advantages such as rapidity, simplicity, and high sensitivity and selectivity, is expected to contribute to the practical routine analysis in forensic and toxicological fields.

Graphical Abstract

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顶空气相色谱-质谱法快速同时测定全血中氰化物、叠氮化物和乙醇

快速检测和定量生物标本中的有毒物质和酒精是当务之急，特别是在非自然死亡和中毒事件中。在这项研究中，我们开发了一种高通量同时分析全血中氰化物、叠氮化物和乙醇的方法。该方法涉及一个简单的手动预处理步骤，需要在全血样本中依次添加内标、抗坏血酸和磷酸（在1分钟内），然后进行顶空气相色谱-质谱分析（在14分钟内）。该方法表现出高线性（相关系数， >； 0.9996），ng/mL水平检测的灵敏度，以及令人满意的精密度（氰化物的相对标准偏差小于5.8%，叠氮化物的相对标准差小于8.8%，乙醇的相对标准误差小于2.9%）和真实度（氰化物、叠氮化物和乙醇的相对误差在7.3%、17%和6.9%以内）。该方法不需要复杂的分析技术，具有快速、简单、高灵敏度和选择性等优点，有望为法医学和毒理学领域的实用常规分析做出贡献。图形摘要

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.