DNA extraction and STR profiling from histological slides

IF 0.5 Q4 GENETICS & HEREDITY

Forensic Science International: Genetics Supplement Series Pub Date : 2022-12-01 DOI:10.1016/j.fsigss.2022.10.026

Viktor Soma Poór , Marah Alhabahbeh , Vivien Fejes , Gábor Kovács , Zsolt Pádár

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引用次数: 1

Abstract

Formalin-fixed paraffin-embedded (FFPE) tissue blocks are commonly used in the field of pathology and forensic pathology as a source of histological slides. For postmortem kinship analysis or identification, DNA can be extracted from blocks with specialized kits. However, when an STR profile should be generated from single microscope slides, the removal of the coverslip and the limited sample size poses unique challenges. We aimed to test the effectivity of agitated xylene incubation to dissolve the mounting material to facilitate the coverslip removal. DNA extraction tests were performed on 5- to 7-year-old histological slides. Xylol was used to dissolve the mounting medium to facilitate cover slide removal, one set of samples was shaken during incubation, and the other set was left still. It was found that shaking the sample while bathed in xylol decreased the incubation time from three days to two days. Agitation not just reduced the processing time but increased the quality of acquired STR profiles: on average 30% more alleles were detected from the shaken samples compared to the still bathed ones.

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组织切片的DNA提取和STR分析

福尔马林固定石蜡包埋（FFPE）组织块通常用于病理学和法医病理学领域，作为组织学切片的来源。为了进行尸检亲属关系分析或鉴定，可以使用专门的试剂盒从块中提取DNA。然而，当STR图谱应该从单个显微镜载玻片中生成时，去除盖玻片和有限的样本量带来了独特的挑战。我们的目的是测试搅拌二甲苯培养溶解安装材料以促进盖玻片去除的有效性。对5至7岁的组织学切片进行DNA提取测试。使用二甲苯溶解固定培养基，以便于移除盖玻片，在培养过程中摇动一组样品，另一组样品保持静止。研究发现，在二甲苯中浸泡时摇动样品可将培养时间从三天缩短到两天。搅拌不仅减少了处理时间，而且提高了获得的STR图谱的质量：与仍然浸泡的样品相比，从摇晃的样品中检测到的等位基因平均多30%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Forensic Science International: Genetics Supplement Series Medicine-Pathology and Forensic Medicine

CiteScore

0.40

自引率

0.00%

发文量

122

审稿时长

25 days

期刊介绍： The Journal of Forensic Science International Genetics Supplement Series is the perfect publication vehicle for the proceedings of a scientific symposium, commissioned thematic issues, or for disseminating a selection of invited articles. The Forensic Science International Genetics Supplement Series is part of a duo of publications on forensic genetics, published by Elsevier on behalf of the International Society for Forensic Genetics.