A comprehensive GlobalFiler™ autosomal STR reference dataset for Southern Africa

IF 0.5 Q4 GENETICS & HEREDITY

Forensic Science International: Genetics Supplement Series Pub Date : 2022-12-01 DOI:10.1016/j.fsigss.2022.10.046

Mohaimin Kasu , Mpasi Lesaoana , Maria Eugenia D’Amato

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引用次数: 0

Abstract

In this study a total of n = 832 autosomal DNA profiles from Southern Africa are analysed using the GlobalFiler™ STR panel. The dataset includes South Africa (SA) profiles (n = 541) produced by Ristow et al. 2016 and includes newly generated data for SA Sepedi (n = 96) and Lesotho populations (n = 195). For the newly generated (n = 291) genotypes, we report a large degree of rare and novel variation. This included (n = 7) off-ladder allele variants and (n = 7) TPOX tri-alleles. We report forensic summary statistics and genetic diversity parameters. Expected heterozygosity and observed heterozygosity ranged between (0.7– 0.9) with SE33 as the most polymorphic and TH01 the least. For SA and Lesotho genotypes the combined match probability was (1.13 ×10^-24 and 6.035 ×10^-24) and the combined paternity index (1.4 ×10⁹ and 2.44 ×10⁸) respectively. The power of exclusion (0.9999) was similar for each dataset and no significant departures from Hardy-Weinberg equilibrium (HWE) were observed after Bonferroni correction. Population comparisons were performed by MDS and neighbour-joining and population structure inferred by STRUCTURE and DAPC unsupervised clustering.

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一个全面的GlobalFiler™常染色体STR参考数据集，用于南部非洲

在这项研究中，使用GlobalFiler分析了来自南部非洲的总共n=832个常染色体DNA图谱™ STR面板。该数据集包括Ristow等人2016年编制的南非（SA）概况（n=541），并包括新生成的南非塞佩迪（n=96）和莱索托人口（n=195）的数据。对于新产生的（n=291）基因型，我们报告了大量罕见和新的变异。这包括（n＝7）个梯外等位基因变体和（n＝8）个TPOX三等位基因。我们报告法医汇总统计数据和遗传多样性参数。预期杂合度和观察到的杂合度在（0.7-0.9）之间，SE33是最多态的，TH01是最小的。SA和莱索托基因型的组合匹配概率分别为（1.13×10-24和6.035×10-24）和组合亲子指数（1.4×109和2.44×108）。每个数据集的排除能力（0.9999）相似，在Bonferroni校正后没有观察到与Hardy-Weinberg平衡（HWE）的显著偏离。通过MDS和邻居连接进行种群比较，并通过structure和DAPC无监督聚类推断种群结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Forensic Science International: Genetics Supplement Series Medicine-Pathology and Forensic Medicine

CiteScore

0.40

自引率

0.00%

发文量

122

审稿时长

25 days

期刊介绍： The Journal of Forensic Science International Genetics Supplement Series is the perfect publication vehicle for the proceedings of a scientific symposium, commissioned thematic issues, or for disseminating a selection of invited articles. The Forensic Science International Genetics Supplement Series is part of a duo of publications on forensic genetics, published by Elsevier on behalf of the International Society for Forensic Genetics.