Development and validation of molecular methodologies for identification of biological traces from crime scenes

IF 0.5 Q4 GENETICS & HEREDITY

Forensic Science International: Genetics Supplement Series Pub Date : 2022-12-01 DOI:10.1016/j.fsigss.2022.10.006

Alessandra S. Dias , Anna Beatriz Rodrigues Gonçalves , Tatiana Lúcia Santos Nogueira , Dayse A. Silva

引用次数: 0

Abstract

This study aimed to develop a methodology to identify biological fluids in sexual assault cases through mRNA markers. Biological fluid samples such as blood, saliva, and semen were collected from volunteers and submitted to RT-qPCR reactions with specific primers for the biomarkers HTN3 (saliva), ALAS (blood) andTGM4 (semen). The Melting (Tm) of each biological fluid was analyzed and the result inferred a high specificity capable of differentiating such traces. Biplex systems were generated to improve trace analysis in a single qPCR reaction.

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从犯罪现场鉴定生物痕迹的分子方法的发展和验证

本研究旨在开发一种通过信使核糖核酸标记物识别性侵病例中生物液体的方法。从志愿者身上采集血液、唾液和精液等生物流体样本，并用生物标志物HTN3（唾液）、ALAS（血液）和TGM4（精液）的特异性引物进行RT-qPCR反应。分析了每种生物流体的熔化（Tm），结果推断出能够区分这些痕迹的高特异性。产生双联系统以改进单一qPCR反应中的痕量分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Forensic Science International: Genetics Supplement Series Medicine-Pathology and Forensic Medicine

CiteScore

0.40

自引率

0.00%

发文量

122

审稿时长

25 days

期刊介绍： The Journal of Forensic Science International Genetics Supplement Series is the perfect publication vehicle for the proceedings of a scientific symposium, commissioned thematic issues, or for disseminating a selection of invited articles. The Forensic Science International Genetics Supplement Series is part of a duo of publications on forensic genetics, published by Elsevier on behalf of the International Society for Forensic Genetics.