Evaluation of genetic markers for the analysis of THC levels of Cannabis sativa samples using principal component analysis – A preliminary study

IF 0.5 Q4 GENETICS & HEREDITY

Forensic Science International: Genetics Supplement Series Pub Date : 2022-12-01 DOI:10.1016/j.fsigss.2022.10.004

S. Cisana , M. Omedei , M. Di Nunzio , F. Seganti , V. Brenzini , A. Coppi , A. Berti , C. Di Nunzio , P. Garofano , E. Alladio

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Abstract

Cannabis sativa is a worldwide commercial plant used for medicinal purposes, food and fiber production, and also as a recreational drug. Therefore, the identification and differentiation between legal and illegal C. sativa is of great importance for forensic investigations. In this study, principal component analysis (PCA), an exploratory data analysis technique, was tested to correlate the specific genotype with the concentration of tetrahydrocannabinol (THC) in the samples. C. sativa samples were obtained from legal growers in Piedmont, Italy, and from illegal drug seizures in the Turin region. DNA was extracted, quantified, amplified with a 13-loci multiplex STR and finally analyzed with an automated sequencer. The results showed a trend in the analyzed samples as they differed by their THC content and allele profiles. PCA yielded two clusters of samples that differed by specific allele profiles and THC concentrations. Further validation studies are needed, but this study could provide a new approach to forensic investigation and be a valuable aid to law enforcement in significant marijuana seizures or in tracing illicit drug trafficking routes.

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利用主成分分析评估大麻样本中四氢大麻酚水平的遗传标记-初步研究

大麻是一种世界性的商业植物，用于药用、食品和纤维生产，也是一种娱乐药物。因此，识别和区分合法和非法的紫花苜蓿对法医学调查具有重要意义。在本研究中，主成分分析（PCA）是一种探索性数据分析技术，旨在将特定基因型与样本中四氢大麻酚（THC）的浓度相关联。C.sativa样本是从意大利皮埃蒙特的合法种植者和都灵地区的非法毒品缉获中获得的。DNA被提取、定量，用13个基因座的多重STR扩增，最后用自动测序仪进行分析。结果显示，分析样本的THC含量和等位基因图谱存在差异，因此呈趋势。PCA产生了两组因特定等位基因图谱和四氢大麻酚浓度而不同的样本。还需要进一步的验证研究，但这项研究可以为法医调查提供一种新的方法，并为执法部门在大量大麻缉获或追踪非法贩毒路线方面提供宝贵的帮助。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Forensic Science International: Genetics Supplement Series Medicine-Pathology and Forensic Medicine

CiteScore

0.40

自引率

0.00%

发文量

122

审稿时长

25 days

期刊介绍： The Journal of Forensic Science International Genetics Supplement Series is the perfect publication vehicle for the proceedings of a scientific symposium, commissioned thematic issues, or for disseminating a selection of invited articles. The Forensic Science International Genetics Supplement Series is part of a duo of publications on forensic genetics, published by Elsevier on behalf of the International Society for Forensic Genetics.