{"title":"Storage of spheroplasts at −70°C for transfection with φX174 RFDNA","authors":"H.V. Malling , M.C. Fater , J.G. Burkhart , S.C. Hardies , C.A. Hutchison III , M.H. Edgell","doi":"10.1016/0735-0651(87)90014-8","DOIUrl":null,"url":null,"abstract":"<div><p>A method was developed for preparation of spheroplasts used for transfection with φX174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at −70°C.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"4 2","pages":"Pages 23-26"},"PeriodicalIF":0.0000,"publicationDate":"1987-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(87)90014-8","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0735065187900148","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
A method was developed for preparation of spheroplasts used for transfection with φX174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at −70°C.
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