{"title":"Characterization of the target cell receptor for IgE—IV.","authors":"D.H. Conrad , A. Froese","doi":"10.1016/0161-5890(78)90086-X","DOIUrl":null,"url":null,"abstract":"<div><p>Complexes, consisting of IgE and its receptor from rat basophilic leukemia (RBL) cells, were isolated by allowing RBL cells to interact with DNP-IgE, solubilizing the cells with Nonidet P-40 and by allowing the extract to bind to anti-DNP-Sepharose. Of the RBL surface material eluted with 2,4-dinitrophenolate, 51–65% was precipitable by anti-IgE. Polyacrylamide gel analysis of eluates in SDS. using 10% gels, revealed a single surface component with an apparent molecular weight of about 45.000 daltons.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 5","pages":"Pages 283-288"},"PeriodicalIF":0.0000,"publicationDate":"1978-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90086-X","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/016158907890086X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 8
Abstract
Complexes, consisting of IgE and its receptor from rat basophilic leukemia (RBL) cells, were isolated by allowing RBL cells to interact with DNP-IgE, solubilizing the cells with Nonidet P-40 and by allowing the extract to bind to anti-DNP-Sepharose. Of the RBL surface material eluted with 2,4-dinitrophenolate, 51–65% was precipitable by anti-IgE. Polyacrylamide gel analysis of eluates in SDS. using 10% gels, revealed a single surface component with an apparent molecular weight of about 45.000 daltons.
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