{"title":"Aldosterone inhibits nitric oxide synthesis in rat vascular smooth muscle cells induced by interleukin-1 β","authors":"Uichi Ikeda , Toshiko Kanbe , Ichiro Nakayama , Yasuhiro Kawahara , Mitsuhiro Yokoyama , Kazuyuki Shimada","doi":"10.1016/0922-4106(95)90018-7","DOIUrl":null,"url":null,"abstract":"<div><p>We investigated the effects of aldosterone on nitric oxide (NO) synthesis in vascular smooth muscle cells. We measured the production of nitrite, a stable metabolite of NO, and the expression of inducible NO synthase mRNA and protein in cultured rat vascular smooth muscle cells. Incubation of the cultures with interleukin-1β (10 ng/ml) for 24 h caused a significant increase in nitrite generation. The interleukin-1β-induced nitrite production by vascular smooth muscle cells was significantly inhibited by aldosterone in a dose (10<sup>−9</sup> ∼ 10<sup>−6</sup> M)-dependent manner. Incubation with interleukin-1β for 12 ∼ 24 h caused inducible NO synthase mRNA expression in vascular smooth muscle cells, whereas aldosterone had a suppressive effect on its expression. Aldosterone also decreased interleukin-1β-induced inducible NO synthase protein accumulation. These results indicate that aldosterone inhibits NO synthesis under interleukin-1β-stimulated conditions in vascular smooth muscle cells.</p></div>","PeriodicalId":100502,"journal":{"name":"European Journal of Pharmacology: Molecular Pharmacology","volume":"290 2","pages":"Pages 69-73"},"PeriodicalIF":0.0000,"publicationDate":"1995-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-4106(95)90018-7","citationCount":"93","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Pharmacology: Molecular Pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0922410695900187","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 93
Abstract
We investigated the effects of aldosterone on nitric oxide (NO) synthesis in vascular smooth muscle cells. We measured the production of nitrite, a stable metabolite of NO, and the expression of inducible NO synthase mRNA and protein in cultured rat vascular smooth muscle cells. Incubation of the cultures with interleukin-1β (10 ng/ml) for 24 h caused a significant increase in nitrite generation. The interleukin-1β-induced nitrite production by vascular smooth muscle cells was significantly inhibited by aldosterone in a dose (10−9 ∼ 10−6 M)-dependent manner. Incubation with interleukin-1β for 12 ∼ 24 h caused inducible NO synthase mRNA expression in vascular smooth muscle cells, whereas aldosterone had a suppressive effect on its expression. Aldosterone also decreased interleukin-1β-induced inducible NO synthase protein accumulation. These results indicate that aldosterone inhibits NO synthesis under interleukin-1β-stimulated conditions in vascular smooth muscle cells.