Challenges and opportunities in elucidating the structures of biofilm exopolysaccharides: A case study of the Pseudomonas aeruginosa exopolysaccharide called Pel

IF 1.9 3区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Kristen Amyx-Sherer, Courtney Reichhardt
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引用次数: 0

Abstract

Biofilm formation protects bacteria from antibiotic treatment and host immune responses, making biofilm infections difficult to treat. Within biofilms, bacterial cells are entangled in a self-produced extracellular matrix that typically includes exopolysaccharides. Molecular-level descriptions of biofilm matrix components, especially exopolysaccharides, have been challenging to attain due to their complex nature and lack of solubility and crystallinity. Solid-state nuclear magnetic resonance (NMR) has emerged as a key tool to determine the structure of biofilm matrix exopolysaccharides without degradative sample preparation. In this review, we discuss challenges of studying biofilm matrix exopolysaccharides and opportunities to develop solid-state NMR approaches to study these generally intractable materials. We specifically highlight investigations of the exopolysaccharide called Pel made by the opportunistic pathogen, Pseudomonas aeruginosa. We provide a roadmap for determining exopolysaccharide structure and discuss future opportunities to study such systems using solid-state NMR. The strategies discussed for elucidating biofilm exopolysaccharide structure should be broadly applicable to studying the structures of other glycans.

Abstract Image

阐明生物膜胞外多糖结构的挑战和机遇:一个名为Pel的铜绿假单胞菌胞外多糖类的案例研究。
生物膜的形成保护细菌免受抗生素治疗和宿主免疫反应的影响,使生物膜感染难以治疗。在生物膜中,细菌细胞被自身产生的细胞外基质缠住,该基质通常包括胞外多糖。生物膜基质成分,特别是胞外多糖,由于其复杂的性质以及缺乏溶解性和结晶性,很难获得分子水平的描述。固态核磁共振(NMR)已成为在没有降解样品制备的情况下确定生物膜基质胞外多糖结构的关键工具。在这篇综述中,我们讨论了研究生物膜基质胞外多糖的挑战,以及开发固态NMR方法来研究这些通常难以处理的材料的机会。我们特别强调了对机会病原体铜绿假单胞菌产生的称为Pel的胞外多糖的研究。我们提供了确定胞外多糖结构的路线图,并讨论了使用固态NMR研究此类系统的未来机会。所讨论的阐明生物膜胞外多糖结构的策略应广泛适用于研究其他聚糖的结构。
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来源期刊
CiteScore
4.70
自引率
10.00%
发文量
99
审稿时长
1 months
期刊介绍: MRC is devoted to the rapid publication of papers which are concerned with the development of magnetic resonance techniques, or in which the application of such techniques plays a pivotal part. Contributions from scientists working in all areas of NMR, ESR and NQR are invited, and papers describing applications in all branches of chemistry, structural biology and materials chemistry are published. The journal is of particular interest not only to scientists working in academic research, but also those working in commercial organisations who need to keep up-to-date with the latest practical applications of magnetic resonance techniques.
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