Exercise training decreases the load and changes the content of circulating SDS-resistant protein aggregates in patients with heart failure with reduced ejection fraction.

IF 3.5 2区生物学 Q3 CELL BIOLOGY

Molecular and Cellular Biochemistry Pub Date : 2024-10-01 Epub Date: 2023-10-30 DOI:10.1007/s11010-023-04884-z

Marisol Gouveia, Cristine Schmidt, Priscilla Gois Basilio, Susana S Aveiro, Pedro Domingues, Ke Xia, Wilfredo Colón, Rui Vitorino, Rita Ferreira, Mário Santos, Sandra I Vieira, Fernando Ribeiro

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Abstract

Background: Heart failure (HF) often disrupts the protein quality control (PQC) system leading to protein aggregate accumulation. Evidence from tissue biopsies showed that exercise restores PQC system in HF; however, little is known about its effects on plasma proteostasis.

Aim: To determine the effects of exercise training on the load and composition of plasma SDS-resistant protein aggregates (SRA) in patients with HF with reduced ejection fraction (HFrEF).

Methods: Eighteen patients with HFrEF (age: 63.4 ± 6.5 years; LVEF: 33.4 ± 11.6%) participated in a 12-week combined (aerobic plus resistance) exercise program (60 min/session, twice per week). The load and content of circulating SRA were assessed using D2D SDS-PAGE and mass spectrometry. Cardiorespiratory fitness, quality of life, and circulating levels of high-sensitive C-reactive protein, N-terminal pro-B-type natriuretic peptide (NT-proBNP), haptoglobin and ficolin-3, were also evaluated at baseline and after the exercise program.

Results: The exercise program decreased the plasma SRA load (% SRA/total protein: 38.0 ± 8.9 to 36.1 ± 9.7%, p = 0.018; % SRA/soluble fraction: 64.3 ± 27.1 to 59.8 ± 27.7%, p = 0.003). Plasma SRA of HFrEF patients comprised 31 proteins, with α-2-macroglobulin and haptoglobin as the most abundant ones. The exercise training significantly increased haptoglobin plasma levels (1.03 ± 0.40 to 1.11 ± 0.46, p = 0.031), while decreasing its abundance in SRA (1.83 ± 0.54 × 10¹¹ to 1.51 ± 0.59 × 10¹¹, p = 0.049). Cardiorespiratory fitness [16.4(5.9) to 19.0(5.2) ml/kg/min, p = 0.002], quality of life, and circulating NT-proBNP [720.0(850.0) to 587.0(847.3) pg/mL, p = 0.048] levels, also improved after the exercise program.

Conclusion: Exercise training reduced the plasma SRA load and enhanced PQC, potentially via haptoglobin-mediated action, while improving cardiorespiratory fitness and quality of life of patients with HFrEF.

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运动训练降低了射血分数降低的心力衰竭患者的负荷，并改变了循环SDS抗性蛋白聚集体的含量。

背景：心力衰竭（HF）经常破坏蛋白质质量控制（PQC）系统，导致蛋白质聚集体积累。来自组织活检的证据表明，运动可以恢复HF患者的PQC系统；然而，人们对其对血浆蛋白稳定的影响知之甚少。目的：研究运动训练对射血分数降低的HF患者血浆SDS抗性蛋白聚集体（SRA）负荷和组成的影响 ± 6.5年；LVEF:33.4 ± 11.6%）参加了为期12周的综合（有氧加阻力）锻炼计划（60分钟/次，每周两次）。使用D2D SDS-PAGE和质谱法评估循环SRA的负载和含量。在基线和运动项目后，还评估了心肺健康、生活质量和高敏C反应蛋白、N-末端B型钠尿肽原（NT-proBNP）、触珠蛋白和ficolin-3的循环水平。结果：运动方案降低了血浆SRA负荷（%SRA/总蛋白：38.0 ± 8.9至36.1 ± 9.7%，p = 0.018；%SRA/可溶性组分：64.3 ± 27.1至59.8 ± 27.7%，p = 0.003）。HFrEF患者的血浆SRA包括31种蛋白质，其中α-2-巨球蛋白和触珠蛋白含量最高。运动训练显著提高了触珠蛋白血浆水平（1.03 ± 0.40至1.11 ± 0.46，p = 0.031），同时降低其在SRA中的丰度（1.83 ± 0.54 × 1011至1.51 ± 0.59 × 1011，p = 0.049）。心肺健康[16.4（5.9）至19.0（5.2）ml/kg/min，p = 0.002]，生活质量和循环NT-proBNP[720.00（850.0）至587.0（847.3）pg/mL，p = 0.048]水平，在运动计划后也有所改善。结论：运动训练可能通过触珠蛋白介导的作用降低了血浆SRA负荷并增强了PQC，同时改善了HFrEF患者的心肺健康和生活质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular and Cellular Biochemistry 生物-细胞生物学

CiteScore

8.30

自引率

2.30%

发文量

293

审稿时长

1.7 months

期刊介绍： Molecular and Cellular Biochemistry: An International Journal for Chemical Biology in Health and Disease publishes original research papers and short communications in all areas of the biochemical sciences, emphasizing novel findings relevant to the biochemical basis of cellular function and disease processes, as well as the mechanics of action of hormones and chemical agents. Coverage includes membrane transport, receptor mechanism, immune response, secretory processes, and cytoskeletal function, as well as biochemical structure-function relationships in the cell. In addition to the reports of original research, the journal publishes state of the art reviews. Specific subjects covered by Molecular and Cellular Biochemistry include cellular metabolism, cellular pathophysiology, enzymology, ion transport, lipid biochemistry, membrane biochemistry, molecular biology, nuclear structure and function, and protein chemistry.