Enzyme-Enhanced Electrochemical Immunoassay for Phenytoin

Abstract

Research Triangle Institute Post Office Box 12194 Research Triangle Park, NC 27709-2194 drug to be effective and safe, plasma levels must be between 5 and 20 mg/L [11]. Thus, the development of easy, real-time assays for this and other critical-level drugs is highly desirable. This paper also describes preliminary results on the electron-transfer mediation of ferrocene derivatives to polypyrrole-immobilized GOx. The goal of these experiments is to couple the polypyrrole-immobilized GOx to the ferrocene-DPH system to produce a reagentless electrochemical immunoassay sensor, so that easy, real-time determinations may be realized. Introduction