Tumour Associated Tissue Eosinophilia in Oral Squamous Cell Carcinoma by Special Histochemical Analysis of Tissue Eosinophilia using Congo Red Staining

R. Sujatha, Rufaida Shafiuddin, M. V. Kulkarni, Y. Manjunatha
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Abstract

Introduction: Tumour Associated Tissue Eosinophilia (TATE) is an important phenomenon occurring in the tumour microenvironment and has a pivotal role. TATE is defined as “eosinophilic stromal infiltration of a tumour not associated with tumour necrosis or ulceration”. The exact role of TATE in malignancies is yet unclear but studies have shown that TATE usually has a favourable outcome in head and neck Squamous Cell Carcinoma (SCC). Therefore, more studies are needed to substantiate this data. Aim: To compare TATE between normal epithelium and Oral Squamous Cell Carcinoma (OSCC) and compare TATE between the histological grades of OSCC. Materials and Methods: A retrospective study was conducted at Dr. BR Ambedkar Medical College, Bangalore, Karnataka, India, from June 2019-January 2021 which included 50 cases, 10 from normal mucosa and 40 histopathologically diagnosed cases of OSCC. 1% congo red solution was used to stain 4 μm thick sections. The sections were examined at a high magnification (40X) and 10 microscopic fields were examined in succession for eosinophils. The average number of eosinophils observed were compared using univariate analysis, which included the Analysis of Variance (ANOVA) test and the Chi- square test and a p-value of 0.001 was considered statistically significant. Results: The mean value of tissue eosinophils increased in OSCC compared to normal mucosa, according to present study findings. When comparing different grades of carcinoma, statistical analysis revealed that well differentiated squamous cell carcinoma had a greater TATE than other grades, which was statistically significant (p-value <0.001). Conclusion: The higher eosinophil count in well differentiated OSCC compared to the other grades could be associated with a better clinical outcome for the patient. In OSCC, TATE can be utilised as a predictor of a good prognosis.
用刚果红染色对口腔鳞状细胞癌组织嗜酸性粒细胞的特殊组织化学分析
肿瘤相关组织嗜酸性粒细胞(tumor Associated Tissue Eosinophilia, TATE)是肿瘤微环境中发生的一种重要现象,具有举足轻重的作用。TATE定义为“肿瘤嗜酸性间质浸润,与肿瘤坏死或溃疡无关”。TATE在恶性肿瘤中的确切作用尚不清楚,但研究表明,TATE在头颈部鳞状细胞癌(SCC)中通常有良好的预后。因此,需要更多的研究来证实这一数据。目的:比较正常上皮与口腔鳞状细胞癌(OSCC)的TATE,并比较OSCC组织学分级之间的TATE。材料和方法:2019年6月至2021年1月,在印度卡纳塔克邦班加罗尔的Dr. BR Ambedkar医学院进行了一项回顾性研究,其中包括50例OSCC, 10例来自正常粘膜,40例组织病理学诊断的OSCC。1%刚果红溶液对4 μm厚的切片进行染色。在高倍显微镜下(40倍)检查切片,并连续检查10个显微镜视野中的嗜酸性粒细胞。观察到的嗜酸性粒细胞的平均数量采用单因素分析进行比较,其中包括方差分析(ANOVA)检验和卡方检验,p值为0.001被认为具有统计学意义。结果:根据目前的研究结果,与正常粘膜相比,组织嗜酸性粒细胞的平均值在OSCC中增加。在比较不同级别的癌时,统计分析显示,高分化鳞状细胞癌的TATE高于其他级别,差异有统计学意义(p值<0.001)。结论:与其他级别相比,高分化OSCC中较高的嗜酸性粒细胞计数可能与患者更好的临床结果相关。在OSCC中,TATE可以作为预后良好的预测指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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