Bioactive Compounds of Fresh and Dried Pleurotus ostreatus Mushroom

E. Mohamed, Fatma A Farghaly
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引用次数: 42

Abstract

Chemical composition of the fresh and dried mushroom Pleurotus ostreatus and their bioactive secondary metabolic products were studied. The ethanolic extracts of the P. ostreatus cultivated on rice straw supplemented by wheat bran were studied by Gas Chromatography/Mass Spectrometry (GC/MS) analysis. A total of hundred and seven metabolites were detected in tested samples. These include 56 metabolites only detected in fresh sample, 37 metabolites only detected in dry sample, and 14 metabolites were detected in the both samples. The detected metabolites could be classified into nine chemical groups including 2 metabolites related to acids, 5 alcohols, 27 alkane, 3 amides, 27 esters, 8 fatty acids, 4 terpenoids, 29 heterocyclic and 2 phenols. The bioactivity of the metabolic products could be classified: anticholesterol, anticancer and essential fatty acids which support human health. On comparison between fresh and dried P. ostreatus samples, we found that the high number of metabolites was recorded in the fresh extract. Fifty five aroma compounds were recorded and including (27 esters, 9 ketones, 7 thiols, 5 alcholos, 4 terpenoids , 2 phenols and 1 aldehyde). The selenium content in P. ostreatus samples was measured by using ACAL –APR -51-00 test methods and showed that the fresh sample has 58.24 mg/kg but the dry sample has 100.31 mg/kg.
鲜、干平菇的生物活性成分研究
对鲜、干蘑菇平菇的化学成分及其次生代谢产物进行了研究。采用气相色谱/质谱联用(GC/MS)分析方法,研究了麦麸辅助栽培稻秆上的白顶霉乙醇提取物。在测试样本中共检测到107种代谢物。其中56种代谢物仅在新鲜样品中检测到,37种代谢物仅在干燥样品中检测到,14种代谢物在两种样品中均检测到。检测到的代谢物可分为9个化学类群,其中酸类代谢物2个,醇类代谢物5个,烷烃类代谢物27个,酰胺类代谢物3个,酯类代谢物27个,脂肪酸代谢物8个,萜类代谢物4个,杂环类代谢物29个,酚类代谢物2个。代谢产物的生物活性可分为抗胆固醇、抗癌和支持人体健康的必需脂肪酸。在鲜鲜和干鲜样品的比较中,我们发现鲜鲜提取物中代谢产物的数量很高。共检测到55种芳香化合物,其中酯类27种,酮类9种,硫醇类7种,醇类5种,萜类4种,酚类2种,醛类1种。采用ACAL - - APR -51-00测试方法测定了黄颡鱼样品中的硒含量,结果表明,新鲜样品的硒含量为58.24 mg/kg,干燥样品的硒含量为100.31 mg/kg。
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