Investigation of cyto-toxicity of an innovative BIOfactor MTA product in different human cells: a pilot study

Abstract

Objectives: The aim of this study was to investigate the cyto-toxicity of Biodentine, Angelus MTA, BIOfactor MTA on 3T3L-1 fibroblast, and Saos-2 osteosarcoma cell lines with different dilution of all products. Material and methods: 3T3-1 fibroblast was applied to stimulate pulp cells in cell viability test. In order to gene rate an osteoblastic cell-like model, only Saos-2 cells were used. MTT test was utilized to measure cell cytotoxicity in different dilution of all MTA products. SPSS package program was applied to perform Kruskal-Wallis, one-way ANOVA and two-way ANOVA tests. Results: According to results, while Biodentine, Angelus MTA and BIOfactor MTA showed cyto-toxic effects in 1 : 1 and 1 : 2 dilutions, they were not cyto-toxic in 1 : 4 and 1 : 8 dilutions. Conclusions: MTA products have similar cyto-toxicity. BIOfactor MTA has a cyto-toxic effect as other similar products.