{"title":"Rapid determination of lactic acid in anaerobic biological\ntreatment process using a portable sensitive lactate biosensor","authors":"D. Darwin","doi":"10.5114/BTA.2019.85320","DOIUrl":null,"url":null,"abstract":"The rapid and practical determination of lactic acid concentration during anaerobic digestion and in acidification samples is extremely important to ensure the digester is running properly and to avoid build-up of lactic acid. Thus, developing a simple and fast method for analyzing lactic acid in anaerobic digestion samples is important. The results of the present study revealed that lactic acid is an intermediate product of anaerobic digestion, which could be quickly measured using a portable lactate biosensor. We obtained linear standard curves from the relationship between various lactic acid concentrations of the standard solution and the lactate biosensor reading, which were tested at different temperatures (30, 45 and 55EC). A high value of coefficient determination, -0.98–0.99, was obtained from all standard curves. This suggests that the evaluated system was accurate, reliable, and reproducible.","PeriodicalId":8999,"journal":{"name":"BioTechnologia","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BioTechnologia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5114/BTA.2019.85320","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 1
Abstract
The rapid and practical determination of lactic acid concentration during anaerobic digestion and in acidification samples is extremely important to ensure the digester is running properly and to avoid build-up of lactic acid. Thus, developing a simple and fast method for analyzing lactic acid in anaerobic digestion samples is important. The results of the present study revealed that lactic acid is an intermediate product of anaerobic digestion, which could be quickly measured using a portable lactate biosensor. We obtained linear standard curves from the relationship between various lactic acid concentrations of the standard solution and the lactate biosensor reading, which were tested at different temperatures (30, 45 and 55EC). A high value of coefficient determination, -0.98–0.99, was obtained from all standard curves. This suggests that the evaluated system was accurate, reliable, and reproducible.
BioTechnologiaAgricultural and Biological Sciences-Plant Science
CiteScore
1.60
自引率
0.00%
发文量
8
审稿时长
8 weeks
期刊介绍:
BIOTECHNOLOGIA – a high standard, peer-reviewed, quarterly magazine, providing a medium for the rapid publication of research reports and review articles on novel and innovative aspects of biotechnology, computational biology and bionanotechnology.