Assessment of one maize hybrid lot uniformity by UPOV morphological and protein markers

Vojka Babić, K. Marković, N. Kravić, M. Babić, J. Srdić, T. Petrović
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引用次数: 1

Abstract

Monitoring genetic uniformity and identity is very important in maize breeding and seed production. Due to practical reasons, molecular and biochemical methods for the determination of genetic purity and uniformity should reliably reflect genetic differences associated with traditional morphological traits, even when they are not agriculturally important. A comparison of genetic purity and uniformity of one maize hybrid lot, based on morphological UPOV markers and the UTLIEF (Ultrathin-layer isoelectric focusing) method is performed in this research. Tested hybrid samples expressed uniform protein markers profiles, but on the other hand, unsatisfactory uniformity of morphological markers in the field, indicating some laches in seed production. Although the UTLIEF method, standardized by ISTA rules, provides enough accurate distinction between F1 seed and self-pollinated maternal seed, a "clean" isozyme or protein profile will not necessarily correlate with morphological homogeneity. It is most likely that the non-uniformity of the tested hybrid lot originates from the non-uniformity of one of the parental lines. Therefore, to establish where the laches occurred, it is necessary to perform post-control tests with reference samples of maize hybrid and parental lines, as well as, the insight into their official descriptions according to the UPOV descriptor. Thus, it is very important to require the deposition of referent samples of hybrids and varieties whose seed production is allowed in Serbia, as well as to have harmonised protocols for conduction of laboratory and field post-control tests.
利用UPOV形态和蛋白质标记评价玉米杂交小区均匀性
遗传一致性和遗传同一性监测在玉米育种和种子生产中具有重要意义。由于实际原因,用于确定遗传纯度和均匀性的分子和生化方法应该可靠地反映与传统形态性状相关的遗传差异,即使它们在农业上并不重要。利用形态UPOV标记和超薄层等电聚焦(UTLIEF)方法对一个玉米杂交种的遗传纯度和均匀性进行了比较。杂交样品表达的蛋白质标记谱一致,但田间形态标记的一致性不理想,表明在制种过程中存在一定的滞后。尽管UTLIEF方法,标准化的ISTA规则,提供了足够准确的区分F1种子和自花授粉的母系种子,“干净”的同工酶或蛋白质谱不一定与形态同质性相关。所测杂交种的不均匀性很可能是由亲本系之一的不均匀性引起的。因此,为了确定懈怠发生的位置,有必要对玉米杂交种和亲本系的参考样本进行控制后测试,并根据UPOV描述符深入了解它们的官方描述。因此,非常重要的是要求存放塞尔维亚允许生产种子的杂种和品种的参考样品,并制定统一的实验室和现场控制后试验的规程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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