Leaf rust resistance genes identification in the spring bread wheat breeding material of the Agricultural Research Institute for South-East Regions of Russia

Q4 Biochemistry, Genetics and Molecular Biology

Ratarstvo i Povrtarstvo Pub Date : 2019-01-01 DOI:10.5937/RATPOV56-20733

E. Gultyaeva, S. Sibikeev, A. Druzhin, E. Shaydayuk

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引用次数: 2

Abstract

Leaf rust caused by Puccinia triticina is a common bread wheat disease in the Volga Region of Russia and breeding for this disease resistance is a priority for the Agricultural Research Institute for South-East Regions wheat program. Knowledge of the effective resistance genes present in the germplasm is relevant when selecting for effective and more durable resistance. P. triticina races with virulence to Lr9, Lr19, Lr26 and with other different virulence combinations and molecular markers of L r genes were used to determine which seedling resistance genes might be present in the 68 bread wheat lines and cultivars. Studies have shown that the effective protection against leaf rust widespread in the Volga Region spring bread wheat cultivars is controlled by Lr6Ag and Lr6Agi+Lr19 genes. In addition, cultivars carry Lr10, Lr19, Lr10+Lr26 genes. It was found that in the studied set of lines the leaf rust resistance is determined by the following Lr-genes and its combinations: 9, 10, 19, 26, 34, 37, 41, Satu, 6Ag. Moreover, usage frequency of Lr19 is 89.5%, Lr10 -40.4%, Lr26 -31.6%, Lr6Ag -21%, Lr28 -3.5%, Lr41 -3.5%, Lr9 - 1.8%, Lr34 -1.8%, Lr37 -1.8%, LrSatu -1.8%. The frequency of two L r-genes combinations is 45.7%, three - 21% and four Lr-genes - 5.3%. Mainly are used such Lr-genes combinations as: Lr19+Lr26 and Lr10+Lr19+Lr26 - 22.8%; Lr19+Lr6Ag -7%. The four Lr-genes combinations has been included Lr10+Lr26+Lr28?+Lr6Ag -1.8%, Lr1+Lr10+Lr26+Lr6Ag -1.8% and Lr10+Lr19+Lr28?+Lr6Ag -1.8%. In addition, the effective Lr19 with nonidentified Lr-genes from cultivar Saratovskaya 57 (L164) and a. elongatum (CI-7-57) combinations has been identified.

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俄罗斯东南地区农业研究所春小麦育种材料中抗叶锈病基因的鉴定

由小麦锈病引起的叶锈病是俄罗斯伏尔加河地区常见的面包小麦病害，对这种疾病的抗性育种是东南地区农业研究所小麦项目的优先事项。在选择有效和更持久的抗性时，对种质中存在的有效抗性基因的了解是相关的。利用对Lr9、Lr19、Lr26等不同毒力组合的小种和lr基因的分子标记，确定了68个面包小麦品系和品种中可能存在的幼苗抗性基因。研究表明，Lr6Ag和Lr6Agi+Lr19基因对伏尔加河地区春小麦品种普遍存在的叶锈病具有有效的防治作用。此外，品种携带Lr10、Lr19、Lr10+Lr26基因。结果表明，在所研究的品系中，抗叶锈病能力由以下lr基因及其组合决定:9、10、19、26、34、37、41、Satu、6Ag。Lr19的使用频率分别为89.5%、Lr10 -40.4%、Lr26 -31.6%、Lr6Ag -21%、Lr28 -3.5%、Lr41 -3.5%、Lr9 -1.8%、Lr34 -1.8%、Lr37 -1.8%、LrSatu -1.8%。2个L - r基因组合的频率为45.7%，3 - 21%，4个L - r基因组合的频率为5.3%。主要采用Lr19+Lr26和Lr10+Lr19+Lr26 - 22.8%的lr基因组合;Lr19 + Lr6Ag -7%。四个lr基因组合包括Lr10+Lr26+Lr28?Lr1+Lr10+Lr26+Lr6Ag -1.8%和Lr10+Lr19+Lr28?+ Lr6Ag -1.8%。此外，还从栽培品种Saratovskaya 57 (L164)和a. elongatum (CI-7-57)组合中鉴定出具有未鉴定lr基因的有效Lr19。

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来源期刊

Ratarstvo i Povrtarstvo Agricultural and Biological Sciences-Soil Science

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

4 weeks