Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia

Q4 Biochemistry, Genetics and Molecular Biology
D. Milošević, M. Ignjatov, A. Marjanović-Jeromela, I. Stanković, Z. Nikolić, G. Tamindžić, B. Krstic
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引用次数: 1

Abstract

In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Sancevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank.
塞尔维亚一种新的芥菜病原菌——萝卜黄病毒的分子特征
2018年,我们采集了来自塞尔维亚Rimski Sancevi和Senta两个不同地区的58份芥菜样品,并采用tas - eltsa检测了芜菁黄病毒(TuYV)、花菜花叶病毒(CaMV)和芜菁花叶病毒(TuMV)的存在。42份检测标本血清学检测到TuYV,而CaMV和TuMV均为阴性。采用蚜虫传播试验,用2株自然感染的TuYV阳性芥菜苗接种5株试验植株。用特异引物TuYVorf0F/TuYVorf0R扩增780 bp的病毒片段,进行病毒种类鉴定。对分离株88Sal (MK144816)和98Bni (MK144817)的RTPCR产物进行测序,并与TuYV的GenBank序列进行比较。塞尔维亚分离株与波兰TuYV分离株(EU022489)的同源性最高。系统发育分析表明,来自塞尔维亚的TuYV分离株与从GenBank检索到的其他TuYV序列聚类。
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来源期刊
Ratarstvo i Povrtarstvo
Ratarstvo i Povrtarstvo Agricultural and Biological Sciences-Soil Science
CiteScore
0.80
自引率
0.00%
发文量
4
审稿时长
4 weeks
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