Diaporthe pseudolongicolla: The new pathogen on soybean seed in Serbia

Abstract

Seed decay is one of the most important diseases of soybean (Glycine max (L.) Merr.) that has a negative impact on the market grade of soybeans. The disease is mainly caused by Diaporthe longicolla, along with other Diaporthe species. Screening of soybean seeds health status in Vojvodina Province, Serbia, showed cultural and morphological variability among isolates identified as D. longicolla. With the use of DNA sequences of internal transcribed spacer (ITS1-5.8S-ITS2) region and partial translation elongation factor 1-alpha (EF1-α), the new species was determined. BLAST analysis showed 100% identity with D. pseudolongicolla (syn. D. novem) that was described in this study and its taxonomic revision is discussed. Pathogenicity trial showed that both species, D. longicolla and D. pseudolongicolla, are highly pathogenic on soybean stem and seed, causing 100% of stem wilting and more than 82% of seed decay.