Research Article Detection of blaZ and mecA genes and antimicrobial susceptibility in Staphylococcus aureus colonizing multipurpose boxes of dentistry students

IF 0.6 Q4 GENETICS & HEREDITY

Genetics and Molecular Research Pub Date : 2023-01-01 DOI:10.4238/gmr19119

C. Andrade, P. P. Orellana, N. Reinoso, L. Gonzalez, K.M. Toledo, P. Velez

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引用次数: 0

Abstract

Cross-contamination between patient and dentist is a real threat that has not been adequately studied. Staphylococcus aureus, through its characteristic genetic plasticity, has managed to develop multiple virulence and antibiotic resistance proteins. The antibiotic susceptibility profile and the presence of the blaZ and mecA genes that encode resistance to penicillin and methicillin, respectively, were analyzed in strains isolated from multipurpose boxes used by dental students at the Catholic University of Cuenca. These boxes are used to transport instruments and material. From the universe of study (249 boxes) 139 samples were obtained from boxes of the students who accepted and signed a consent to participate. Eight strains of S. aureus were identified, of which, through antibiogram analysis, it was found that seven were resistant to penicillin and two strains resistant to cefoxitin (MRSA strains). In molecular analysis, the mecA gene was identified in two strains, while the blaZ gene was found in all of them. It was concluded that the rate of S. aureus found in this study was low due to various factors, possibly including increased vigilance and cleanliness due to the COVID-19 pandemic during the study. © FUNPEC-RP www.funpecrp.com.br.

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研究文章牙科学生多功能箱金黄色葡萄球菌菌落blaZ和mecA基因检测及药敏

病人和牙医之间的交叉污染是一种真正的威胁，但尚未得到充分的研究。金黄色葡萄球菌，通过其特有的遗传可塑性，已经成功地发展出多种毒力和抗生素抗性蛋白。对从昆卡天主教大学牙科专业学生使用的多用途箱中分离的菌株进行了抗生素敏感性分析，并分别分析了编码青霉素和甲氧西林抗性的blaZ和mecA基因的存在。这些箱子用来运输仪器和材料。从整个研究范围(249个盒子)中，从接受并签署了参与同意书的学生的盒子中获得了139个样本。共鉴定出8株金黄色葡萄球菌，其中经抗菌谱分析，发现7株对青霉素耐药，2株对头孢西丁耐药(MRSA菌株)。分子分析结果显示，2株菌株均含有mecA基因，3株菌株均含有blaZ基因。综上所述，本研究中金黄色葡萄球菌的检出率较低，原因多种多样，可能包括研究期间因COVID-19大流行而提高了警惕和清洁程度。©FUNPEC-RP www.funpecrp.com.br。

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来源期刊

Genetics and Molecular Research 生物-生化与分子生物学

CiteScore

1.00

自引率

25.00%

发文量

审稿时长

3 months

期刊介绍： Genetics and Molecular Research (GMR), maintained by the Research Foundation of Ribeirão Preto (Fundação de Pesquisas Científicas de Ribeirão Preto), publishes high quality research in genetics and molecular biology. GMR reflects the full breadth and interdisciplinary nature of this research by publishing outstanding original contributions in all areas of biology. GMR publishes human studies, as well as research on model organisms—from mice and flies, to plants and bacteria. Our emphasis is on studies of broad interest that provide significant insight into a biological process or processes. Topics include, but are not limited to gene discovery and function, population genetics, evolution, genome projects, comparative and functional genomics, molecular analysis of simple and complex genetic traits, cancer genetics, medical genetics, disease biology, agricultural genomics, developmental genetics, regulatory variation in gene expression, pharmacological genomics, evolution, gene expression, chromosome biology, and epigenetics.