Ub Combination Enhanced Cellular Immune Response Elicited by HSP65 DNA Vaccine against Mycobacterium tuberculosis

Qing-min Wang, Chengxiang Lei, Qiuhong Liu
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引用次数: 3

Abstract

This study observed the immune response induced by a HSP65 DNA vaccine fused with UbGR against Mycobacterium tuberculosis. BALB/c mice were inoculated with HSP65 DNA vaccine, UbGR-fused HSP65 DNA vaccine (Ub-GR-HSP65) and blank vector respectively. HSP65 DNA vaccine elicited a Thl-polarized immune response. The Thl-type cytokine (IFN-γ) and proliferative T cell responses from spleen were improved significantly in UbGR-HSP65 group, compared with those in HSP65 DNA vaccine group. Furthermore, this fusion DNA vaccine also led to an increased ratio of IgG2ato IgGl and the cytotoxicity of T cells. IFN-γ intracellular staining of splenocytes indicated that UbGR-HSP65 fusion DNA vaccine could activate CD4+ and CD8+ T cells, with much higher CD8+ T cells. Thus, this study demonstrated that the UbGR fusion could improve HSP65-specific cellular immune responses, which is helpful to protect against TB infection.
结核分枝杆菌HSP65 DNA疫苗诱导Ub联合增强细胞免疫应答
本研究观察了HSP65 DNA疫苗与UbGR融合对结核分枝杆菌的免疫反应。分别用HSP65 DNA疫苗、ubgr融合HSP65 DNA疫苗(Ub-GR-HSP65)和空白载体接种BALB/c小鼠。HSP65 DNA疫苗引起了thl极化的免疫反应。与HSP65 DNA疫苗组相比,UbGR-HSP65组脾脏thl型细胞因子(IFN-γ)和增殖T细胞应答显著提高。此外,这种融合DNA疫苗还导致igg2to IgGl的比例增加和T细胞的细胞毒性。脾细胞内IFN-γ染色表明,UbGR-HSP65融合DNA疫苗能激活CD4+和CD8+ T细胞,且CD8+ T细胞活性显著提高。因此,本研究表明UbGR融合可以改善hsp65特异性细胞免疫反应,有助于预防结核感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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