The CRISPR-Cas9 System: A Powerful Tool for Genome Engineering and Regulation

Abstract

In this post-genome era of advanced high-throughput DNA/RNA sequencing technologies, information may no longer be a bottleneck to understand and tackle complicated genetic diseases such as cancer. What is still lacking, however, is an efficient, reliable, and easy tool to precisely modify the cellular genome for functional genome annotation, disease modeling, and possibly even corrective gene therapy.