Modified, Rapid, and Accurate Method for the Quantification of Mucopolysaccharides in Urine

Abstract

A modified colorimetric method for the determination of urinary mucopolysaccharides is presented. It involves reaction with Alcian blue dye under relatively mild conditions and reading in the visible spectrophotometry at 608 nm. It is more sensitive than the original method as it measures all classes of urinary mucopolysaccharides. Results in normal children at different ages corrected to creatinine excretion are reported